Wladek Minor

Education

  • BS, University of Warsaw, Poland
  • MS, University of Warsaw, Poland
  • PhD, University of Warsaw, Poland

Primary Appointment

  • Professor, Molecular Physiology and Biological Physics

Contact

Research Interest(s)

Structure-Function Relationships in Proteins; Structural Genomics; Bioinformatics

Research Description

Our laboratory studies macromolecular structure with an aim toward in-depth understanding of structure-function relationships. X-ray diffraction analysis is our primary research tool, but we also employ other physical and biochemical methods of analysis. The program emphasizes two broad themes: crystallographic studies on molecules of immediate interest, and methodology development. Most of the macromolecules that we have under study relate to one or more of a few broad biological areas: cellular signal transduction and metalloproteins. The same systems have been chosen as subjects for methodology development. The methodology development includes the development of various crystallographic tools that create the HKL package. Another research area is high-throughout crystallography and structural genomics. Our lab is a member of Midwestern Center for Structural Genomics Consortium. We develop high-throughput crystallography equipment - integrated system for data collection and analysis has been installed at X9B beamline at NSLS.

Selected Publications

  • Majorek K, Kuhn M, Chruszcz M, Anderson W, Minor W. Structural, functional, and inhibition studies of a Gcn5-related N-acetyltransferase (GNAT) superfamily protein PA4794: a new C-terminal lysine protein acetyltransferase from pseudomonas aeruginosa. The Journal of biological chemistry. 2013;288(42): 30223-35. PMID: 24003232
  • Chruszcz M, Chapman M, Osinski T, Solberg R, Demas M, Porebski P, Majorek K, Pomés A, Minor W. Alternaria alternata allergen Alt a 1: a unique β-barrel protein dimer found exclusively in fungi. The Journal of allergy and clinical immunology. 2012;130(1): 241-7.e9. PMID: 22664167 | PMCID: PMC3391610
  • Chruszcz M, Pomés A, Glesner J, Vailes L, Osinski T, Porebski P, Majorek K, Heymann P, Platts-Mills T, Minor W, Chapman M. Molecular determinants for antibody binding on group 1 house dust mite allergens. The Journal of biological chemistry. 2012;287(10): 7388-98. PMID: 22210776 | PMCID: PMC3293536
  • Davis G, Kong Y, Paige M, Li Z, Merrick E, Hansen T, Suy S, Wang K, Dakshanamurthy S, Cordova A, McManus O, Williams B, Chruszcz M, Minor W, Patel M, Brown M. Asymmetric synthesis and evaluation of a hydroxyphenylamide voltage-gated sodium channel blocker in human prostate cancer xenografts. Bioorganic & medicinal chemistry. 2012;20(6): 2180-8. PMID: 22364743
  • Goral A, Tkaczuk K, Chruszcz M, Kagan O, Savchenko A, Minor W. Crystal structure of a putative isochorismatase hydrolase from Oleispira antarctica. Journal of structural and functional genomics. 2012;13(1): 27-36. PMID: 22350524 | PMCID: PMC3328404
  • Gront D, Grabowski M, Zimmerman M, Raynor J, Tkaczuk K, Minor W. Assessing the accuracy of template-based structure prediction metaservers by comparison with structural genomics structures. Journal of structural and functional genomics. 2012. PMID: 23086054
  • Hou J, Wojciechowska K, Zheng H, Chruszcz M, Cooper D, Cymborowski M, Skarina T, Gordon E, Luo H, Savchenko A, Minor W. Structure of a short-chain dehydrogenase/reductase from Bacillus anthracis. Acta crystallographica. Section F, Structural biology and crystallization communications. 2012;68 632-7. PMID: 22684058 | PMCID: PMC3370898
  • Knapik A, Petkowski J, Otwinowski Z, Cymborowski M, Cooper D, Majorek K, Chruszcz M, Krajewska W, Minor W. A multi-faceted analysis of RutD reveals a novel family of α/β hydrolases. Proteins. 2012;80(10): 2359-68. PMID: 22641504 | PMCID: PMC3459670
  • Majorek K, Porebski P, Dayal A, Zimmerman M, Jablonska K, Stewart A, Chruszcz M, Minor W. Structural and immunologic characterization of bovine, horse, and rabbit serum albumins. Molecular immunology. 2012;52(3): 174-82. PMID: 22677715 | PMCID: PMC3401331
  • Niedzialkowska E, Wang F, Porebski P, Minor W, Higgins J, Stukenberg P. Molecular basis for phosphospecific recognition of histone H3 tails by Survivin paralogues at inner centromeres. Molecular biology of the cell. 2012;23(8): 1457-66. PMID: 22357620 | PMCID: PMC3327328
  • Petkowski J, Schaner Tooley C, Anderson L, Shumilin I, Balsbaugh J, Shabanowitz J, Hunt D, Minor W, Macara I. Substrate Specificity of Mammalian N-Terminal α-Amino Methyltransferase NRMT. Biochemistry. 2012;51(30): 5942-50. PMID: 22769851 | PMCID: PMC3447998
  • Porebski P, Klimecka M, Chruszcz M, Nicholls R, Murzyn K, Cuff M, Xu X, Cymborowski M, Murshudov G, Savchenko A, Edwards A, Minor W. Structural characterization of Helicobacter pylori dethiobiotin synthetase reveals differences between family members. The FEBS journal. 2012. PMID: 22284390 | PMCID: PMC3392494
  • Shabalin I, Porebski P, Cooper D, Grabowski M, Onopriyenko O, Grimshaw S, Savchenko A, Chruszcz M, Minor W. Structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni at 2.7 Å resolution. Acta crystallographica. Section F, Structural biology and crystallization communications. 2012;68 1018-24. PMID: 22949186 | PMCID: PMC3433189
  • Shumilin I, Cymborowski M, Chertihin O, Jha K, Herr J, Lesley S, Joachimiak A, Minor W. Identification of unknown protein function using metabolite cocktail screening. Structure (London, England : 1993). 2012;20(10): 1715-25. PMID: 22940582 | PMCID: PMC3472112
  • Zheng H, Filippova E, Tkaczuk K, Dworzynski P, Chruszcz M, Porebski P, Wawrzak Z, Onopriyenko O, Kudritska M, Grimshaw S, Savchenko A, Anderson W, Minor W. Crystal structures of putative phosphoglycerate kinases from B. anthracis and C. jejuni. Journal of structural and functional genomics. 2012;13(1): 15-26. PMID: 22403005
  • Chruszcz M, Maleki S, Majorek K, Demas M, Bublin M, Solberg R, Hurlburt B, Ruan S, Mattison C, Mattisohn C, Breiteneder H, Minor W. Structural and immunologic characterization of Ara h 1, a major peanut allergen. The Journal of biological chemistry. 2011;286(45): 39318-27. PMID: 21917921 | PMCID: PMC3234756
  • Cooper D, Porebski P, Chruszcz M, Minor W. X-ray crystallography: Assessment and validation of protein-small molecule complexes for drug discovery. Expert opinion on drug discovery. 2011;6(8): 771-782. PMID: 21779303 | PMCID: PMC3138648
  • Filippova E, Chruszcz M, Cymborowski M, Gu J, Savchenko A, Edwards A, Minor W. Crystal structure of a putative transcriptional regulator SCO0520 from Streptomyces coelicolor A3(2) reveals an unusual dimer among TetR family proteins. Journal of structural and functional genomics. 2011;12(3): 149-57. PMID: 21625866
  • Klimecka M, Chruszcz M, Font J, Skarina T, Shumilin I, Onopryienko O, Porebski P, Cymborowski M, Zimmerman M, Hasseman J, Glomski I, Lebioda L, Savchenko A, Edwards A, Minor W. Structural analysis of a putative aminoglycoside N-acetyltransferase from Bacillus anthracis. Journal of molecular biology. 2011;410(3): 411-23. PMID: 21601576 | PMCID: PMC3131501
  • Chruszcz M, Borek D, Domagalski M, Otwinowski Z, Minor W. X-ray diffraction experiment--the last experiment in the structure elucidation process. Advances in protein chemistry and structural biology. 2010;77 23-40. PMID: 20663480 | PMCID: PMC3128764
  • Koclega K, Chruszcz M, Zimmerman M, Bujacz G, Minor W. 'Hot' macromolecular crystals. Crystal growth & design. 2010;10(2): 580. PMID: 20161694 | PMCID: PMC2809425
  • Sledz P, Zheng H, Murzyn K, Chruszcz M, Zimmerman M, Chordia M, Joachimiak A, Minor W. New surface contacts formed upon reductive lysine methylation: improving the probability of protein crystallization. Protein science : a publication of the Protein Society. 2010;19(7): 1395-404. PMID: 20506323 | PMCID: PMC2974831
  • Tooley C, Petkowski J, Muratore-Schroeder T, Balsbaugh J, Shabanowitz J, Sabat M, Minor W, Hunt D, Macara I. NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein. Nature. 2010;466(7310): 1125-8. PMID: 20668449 | PMCID: PMC2939154
  • Chruszcz M, Zimmerman M, Wang S, Koclega K, Zheng H, Evdokimova E, Kudritska M, Cymborowski M, Savchenko A, Edwards A, Minor W. Function-biased choice of additives for optimization of protein crystallization - the case of the putative thioesterase PA5185 from Pseudomonas aeruginosa PAO1. Crystal growth & design. 2009;8(11): 4054-4061. PMID: 19898606 | PMCID: PMC2700756
  • Grabowski M, Chruszcz M, Zimmerman M, Kirillova O, Minor W. Benefits of structural genomics for drug discovery research. Infectious disorders drug targets. 2009;9(5): 459-74. PMID: 19594422 | PMCID: PMC2866842
  • Chinigo G, Paige M, Grindrod S, Hamel E, Dakshanamurthy S, Chruszcz M, Minor W, Brown M. Asymmetric synthesis of 2,3-dihydro-2-arylquinazolin-4-ones: methodology and application to a potent fluorescent tubulin inhibitor with anticancer activity. Journal of medicinal chemistry. 2008;51(15): 4620-31. PMID: 18610995 | PMCID: PMC2584442
  • Chruszcz M, Potrzebowski W, Zimmerman M, Grabowski M, Zheng H, Lasota P, Minor W. Analysis of solvent content and oligomeric states in protein crystals--does symmetry matter? Protein science : a publication of the Protein Society. 2008;17(4): 623-32. PMID: 18359856 | PMCID: PMC2271157
  • Chruszcz M, Wlodawer A, Minor W. Determination of protein structures--a series of fortunate events. Biophysical journal. 2008;95(1): 1-9. PMID: 18441029 | PMCID: PMC2426657
  • Jha K, Shumilin I, Digilio L, Chertihin O, Zheng H, Schmitz G, Visconti P, Flickinger C, Minor W, Herr J. Biochemical and structural characterization of apolipoprotein A-I binding protein, a novel phosphoprotein with a potential role in sperm capacitation. Endocrinology. 2008;149(5): 2108-20. PMID: 18202122 | PMCID: PMC2329272
  • Sasaki T, Maier B, Koclega K, Chruszcz M, Gluba W, Stukenberg P, Minor W, Scrable H. Phosphorylation regulates SIRT1 function. PloS one. 2008;3(12): e4020. PMID: 19107194 | PMCID: PMC2602979
  • Zheng H, Chruszcz M, Lasota P, Lebioda L, Minor W. Data mining of metal ion environments present in protein structures. Journal of inorganic biochemistry. 2008;102(9): 1765-76. PMID: 18614239 | PMCID: PMC2872550
  • Kirillova O, Minor W. Map2mod--a server for evaluation of crystallographic models and their agreement with electron density maps. Bioinformatics (Oxford, England). 2006;22(13): 1660-1. PMID: 16644793
  • Liu Y, Cheney M, Gaudet J, Chruszcz M, Lukasik S, Sugiyama D, Lary J, Cole J, Dauter Z, Minor W, Speck N, Bushweller J. The tetramer structure of the Nervy homology two domain, NHR2, is critical for AML1/ETO's activity. Cancer cell. 2006;9(4): 249-60. PMID: 16616331
  • Minor W, Cymborowski M, Otwinowski Z, Chruszcz M. HKL-3000: the integration of data reduction and structure solution--from diffraction images to an initial model in minutes. Acta crystallographica. Section D, Biological crystallography. 2006;62 859-66. PMID: 16855301