William H. Guilford

Education

  • BS, Saint Francis College
  • PhD, University of Arizona
  • Postdoc, University of Vermont

Primary Appointment

  • Associate Professor, Biomedical Engineering

Contact

Research Interest(s)

Molecular Mechanics of Muscle Contraction and Cell Movement

Research Description

Our goal is to understand the molecular mechanisms by which cells move, with particular emphasis on muscle contraction. We examine the mechanics of these processes at the level of individual molecules using laser traps, fluorescence microscopy, and reconstituted motile and adhesive systems. These allow us to better define the molecular underpinnings of many cell movements, and the molecular basis of many diseases.

Selected Publications

  • Clobes A, Guilford W. Loop 2 of myosin is a force-dependent inhibitor of the rigor bond. Journal of muscle research and cell motility. 2014;35(2): 143-52. PMID: 24500136 | PMCID: PMC4096411
  • Guilford W, Bloodgood R. Laser trap measurements of flagellar membrane motility. Methods in enzymology. 2013;525 85-107. PMID: 23522466
  • Judge J, Stukenborg G, Johnston W, Guilford W, Slingluff C, Hallowell P. Design, Development, and Evaluation of a Novel Retraction Device for Gallbladder Extraction During Laparoscopic Cholecystectomy. Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract. 2013. PMID: 23897085
  • Reed Z, Guilford W, Park J. Thermal denaturation of tilapia myosin and its subunits as affected by constantly increasing temperature. Journal of food science. 2012;76(7): C1018-24. PMID: 22417538
  • Rao V, Clobes A, Guilford W. Force spectroscopy reveals multiple "closed states" of the muscle thin filament. The Journal of biological chemistry. 2011;286(27): 24135-41. PMID: 21597115 | PMCID: PMC3129194
  • Evangelista A, Rao V, Filo A, Marozkina N, Doctor A, Jones D, Gaston B, Guilford W. Direct regulation of striated muscle myosins by nitric oxide and endogenous nitrosothiols. PloS one. 2010;5(6): e11209. PMID: 20585450 | PMCID: PMC2887846
  • Python J, Wilson K, Snook J, Guo B, Guilford W. The viscoelastic properties of microvilli are dependent upon the cell-surface molecule. Biochemical and biophysical research communications. 2010;397(3): 621-5. PMID: 20570653 | PMCID: PMC2907673
  • Snook J, Guilford W. The Effects of Load on E-Selectin Bond Rupture and Bond Formation. Cellular and molecular bioengineering. 2010;3(2): 128-138. PMID: 20526425 | PMCID: PMC2880517
  • Experimental case studies to engage higher cognitive skills. Advances in physiology education. 2009;33(4): 358-9. PMID: 19948689 | PMCID: PMC3776424
  • Laib J, Marin J, Bloodgood R, Guilford W. The reciprocal coordination and mechanics of molecular motors in living cells. Proceedings of the National Academy of Sciences of the United States of America. 2009;106(9): 3190-5. PMID: 19221028 | PMCID: PMC2651315
  • Guilford W, Downs K, Royce T. Knowledge of prenatal health care among Costa Rican and Panamanian women. Revista panamericana de salud p├║blica = Pan American journal of public health. 2008;23(6): 369-76. PMID: 18644204
  • Rao V, Marongelli E, Guilford W. Phosphorylation of tropomyosin extends cooperative binding of myosin beyond a single regulatory unit. Cell motility and the cytoskeleton. 2008;66(1): 10-23. PMID: 18985725 | PMCID: PMC2770177
  • Paschall C, Guilford W, Lawrence M. Enhancement of L-selectin, but not P-selectin, bond formation frequency by convective flow. Biophysical journal. 2007;94(3): 1034-45. PMID: 17890384 | PMCID: PMC2186251
  • Rao V, La Bonte L, Xu Y, Yang Z, French B, Guilford W. Alterations to myofibrillar protein function in nonischemic regions of the heart early after myocardial infarction. American journal of physiology. Heart and circulatory physiology. 2007;293(1): H654-9. PMID: 17400716
  • Snook J, Li J, Helmke B, Guilford W. Peroxynitrite inhibits myofibrillar protein function in an in vitro assay of motility. Free radical biology & medicine. 2007;44(1): 14-23. PMID: 18045543 | PMCID: PMC2180163
  • Guo B, Guilford W. Mechanics of actomyosin bonds in different nucleotide states are tuned to muscle contraction. Proceedings of the National Academy of Sciences of the United States of America. 2006;103(26): 9844-9. PMID: 16785439 | PMCID: PMC1502541
  • "Shrink wrapping" lectures: teaching cell and molecular biology within the context of human pathologies. Cell biology education. 2005;4(2): 138-42. PMID: 15917872 | PMCID: PMC1103715
  • Guilford W, Tournas J, Dascalu D, Watson D. Creating multiple time-shared laser traps with simultaneous displacement detection using digital signal processing hardware. Analytical biochemistry. 2004;326(2): 153-66. PMID: 15003556
  • Guo B, Guilford W. The tail of myosin reduces actin filament velocity in the in vitro motility assay. Cell motility and the cytoskeleton. 2004;59(4): 264-72. PMID: 15505809
  • Rinko L, Lawrence M, Guilford W. The molecular mechanics of P- and L-selectin lectin domains binding to PSGL-1. Biophysical journal. 2003;86(1): 544-54. PMID: 14695299 | PMCID: PMC1303823
  • Teaching peer review and the process of scientific writing. Advances in physiology education. 2002;25(1): 167-75. PMID: 11824193
  • Cheezum M, Walker W, Guilford W. Quantitative comparison of algorithms for tracking single fluorescent particles. Biophysical journal. 2001;81(4): 2378-88. PMID: 11566807 | PMCID: PMC1301708
  • Guilford W, Warshaw D. The molecular mechanics of smooth muscle myosin. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 1998;119(3): 451-8. PMID: 9734329
  • Dupuis D, Guilford W, Wu J, Warshaw D. Actin filament mechanics in the laser trap. Journal of muscle research and cell motility. 1997;18(1): 17-30. PMID: 9147990
  • Guilford W, Dupuis D, Kennedy G, Wu J, Patlak J, Warshaw D. Smooth muscle and skeletal muscle myosins produce similar unitary forces and displacements in the laser trap. Biophysical journal. 1997;72(3): 1006-21. PMID: 9138552 | PMCID: PMC1184489