Victor E. Laubach
- BA,BS, Pennsylvania State University, University Park, PA
- PhD, George Washington University School of Medicine
- Professor, Surgery
- Phone: 434-924-2927
- Email: email@example.com
- Website: http://www.medicine.virginia.edu/clinical/departments/surgery/research/tcv-lab
Translational research on mechanisms and prevention of lung ischemia-reperfusion (IR) injury following transplantation
My laboratory conducts translational studies into mechanisms and prevention of lung ischemia-reperfusion (IR) injury following transplantation. IR injury is a primary cause of primary graft dysfunction and remains a significant and perplexing cause of morbidity and mortality after transplant. IR injury also leads to an increased risk for chronic graft dysfunction and rejection. Our research utilizes both in vivo and in vitro models to explore inflammatory signaling pathways in innate immune cells, epithelial cells and endothelial cells as well as cross-talk among these cells.
Current Research Projects:
T cell-mediated IR injury. We have recently described a paradigm shift in our understanding of the role of T cells in lung IR injury by showing that the activation of invariant natural killer T (iNKT) cells is a key initiator of IR injury by producing IL-17, a key chemokine for the infiltration of neutrophils that contribute to tissue damage. We are continuing to explore the role of iNKT cells and their interaction with alveolar epithelial cells, macrophages and endothelial cells.
NADPH oxidase. We have shown that NADPH oxidase (NOX2)-generated reactive oxygen species is a key component of immune cell activation during IR, and current studies are aimed at testing the hypothesis that NOX2 is a key mechanism for the activation of iNKT cells and macrophages after IR.
Macrophage-mediated IR injury. We have demonstrated an important role for alveolar macrophages and macrophage-derived TNF-alpha in lung IR injury. We are continuing to explore the role of alveolar macrophages as well as their cross-talk with alveolar epithelial cells and iNKT cells.
RAGE/HMGB1 axis. We have shown that macrophage-derived HMGB1 mediates lung IR injury by binding RAGE on iNKT cells and inducing IL-17 production, and we are continuing to study these signaling mechanisms. One potential mechanism being explored is whether RAGE-mediated IL-17 production requires NOX2 activation. In addition, we are exploring how alveolar macrophages release HMGB1 and whether this involves Toll-like Receptor (TLR) signaling in an autocrine fashion.
Adenosine 2A receptor (A2AR) activation. We have shown that A2AR agonists potently attenuate lung IR injury. Data suggest that A2ARs on iNKT cells are largely responsible for the protective effects of A2AR agonists. These effects may entail the inactivation of NOX2 to block IL-17 production by iNKT cells. Ongoing studies are aimed at further defining mechanisms responsible for the anti-inflammatory effects of A2AR agonists.
Ex vivo lung perfusion (EVLP). An exciting new project in our laboratory involves the implementation of a novel technique called ex vivo lung perfusion (EVLP). EVLP is a method of ex vivo perfusion of an questionable (marginal) donor lung with Steen Solution in order to assess its function and to potentially rehabilitate the donor lung for successful transplantation. Most donor lungs (>80%) are rejected for transplantation for various reasons, and the successful application of EVLP to rehabilitate these lungs could drastically increase the donor lung pool size and save more lives of those patients on the transplant waiting list. We are utilizing EVLP in several experimental models including murine, porcine and even marginal human donor lungs. EVLP provides an ideal platform to apply various therapeutic strategies to the isolated organ to enhance rehabilitation of donor lungs. Our laboratory is currently applying the use of an A2AR agonist during EVLP to rehabilitate non-heart beating donor (NHBD) lungs and prevent IR injury.
Models used in the lab:
We heavily utilize a mouse model of IR injury involving various knockout, transgenic and chimeric mice, immune cell ablation studies and adoptive transfer studies. We also utilize an in vitro model of IR using various pulmonary and immune cells, in isolated or co-culture conditions, to answer specific questions about cellular signaling after IR. Finally, our laboratory utilizes a large animal (porcine) model of IR injury, EVLP and lung transplantation as a pre-clinical model.
Techniques used in the lab:
Routine techniques we use in the laboratory include cell culture, purification of primary cells, quantitative RT-PCR, flow cytometry, FACS, ELISA, multiplex (bead-array) cytokine assay, Western blot, ELISPOT assay, gene array, immunohistochemistry, biochemical assays, confocal microscopy and oxidative stress evaluation. Some murine methods we use include thoracic surgery, bone marrow transplantation, immune cell ablation, adoptive transfer of immune cells, pulmonary function measurements using an isolated mouse lung system, bronchoalveolar lavage, and measurement of pulmonary microvascular permeability.
- Sharma A, LaPar D, Stone M, Zhao Y, Kron I, Laubach V. Receptor for advanced glycation end products (RAGE) on iNKT cells mediates lung ischemia-reperfusion injury. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons. 2013;13(9): 2255-67. PMID: 23865790
- Sharma A, Mulloy D, Le L, Laubach V. NADPH oxidase mediates synergistic effects of IL-17 and TNF-α on CXCL1 expression by epithelial cells after lung ischemia-reperfusion. American journal of physiology. Lung cellular and molecular physiology. 2013;306(1): L69-79. PMID: 24186876
- Mulloy D, Stone M, Crosby I, Lapar D, Sharma A, Webb D, Lau C, Laubach V, Kron I. Ex vivo rehabilitation of non-heart-beating donor lungs in preclinical porcine model: delayed perfusion results in superior lung function. The Journal of thoracic and cardiovascular surgery. 2012;144(5): 1208-15. PMID: 22944084 | PMCID: PMC3477251
- Emaminia A, Lapar D, Zhao Y, Steidle J, Harris D, Laubach V, Linden J, Kron I, Lau C. Adenosine A₂A agonist improves lung function during ex vivo lung perfusion. The Annals of thoracic surgery. 2011;92(5): 1840-6. PMID: 22051279 | PMCID: PMC3259746
- Lapar D, Hajzus V, Zhao Y, Lau C, French B, Kron I, Sharma A, Laubach V. Acute hyperglycemic exacerbation of lung ischemia-reperfusion injury is mediated by receptor for advanced glycation end-products signaling. American journal of respiratory cell and molecular biology. 2011;46(3): 299-305. PMID: 21980055 | PMCID: PMC3326427
- Sharma A, LaPar D, Zhao Y, Li L, Lau C, Kron I, Iwakura Y, Okusa M, Laubach V. Natural killer T cell-derived IL-17 mediates lung ischemia-reperfusion injury. American journal of respiratory and critical care medicine. 2011;183(11): 1539-49. PMID: 21317314 | PMCID: PMC3137143
- Gazoni L, Walters D, Unger E, Linden J, Kron I, Laubach V. Activation of A1, A2A, or A3 adenosine receptors attenuates lung ischemia-reperfusion injury. The Journal of thoracic and cardiovascular surgery. 2010;140(2): 440-6. PMID: 20398911
- Laubach V, French B, Okusa M. Targeting of adenosine receptors in ischemia-reperfusion injury. Expert opinion on therapeutic targets. 2010;15(1): 103-18. PMID: 21110787 | PMCID: PMC3075429
- Laubach V, Kron I. Pulmonary inflammation after lung transplantation. Surgery. 2009;146(1): 1-4. PMID: 19541003
- Sharma A, Linden J, Kron I, Laubach V. Protection from pulmonary ischemia-reperfusion injury by adenosine A2A receptor activation. Respiratory research. 2009;10 58. PMID: 19558673
- Yang Z, Sharma A, Linden J, Kron I, Laubach V. CD4+ T lymphocytes mediate acute pulmonary ischemia-reperfusion injury. The Journal of thoracic and cardiovascular surgery. 2009;137(3): 695-702; discussion 702. PMID: 19258091
- Yang Z, Sharma A, Marshall M, Kron I, Laubach V. NADPH oxidase in bone marrow-derived cells mediates pulmonary ischemia-reperfusion injury. American journal of respiratory cell and molecular biology. 2008;40(3): 375-81. PMID: 18787174
- Sharma A, Fernandez L, Awad A, Kron I, Laubach V. Proinflammatory response of alveolar epithelial cells is enhanced by alveolar macrophage-produced TNF-alpha during pulmonary ischemia-reperfusion injury. American journal of physiology. Lung cellular and molecular physiology. 2007;293(1): L105-13. PMID: 17416740
- Zhao M, Fernandez L, Doctor A, Sharma A, Zarbock A, Tribble C, Kron I, Laubach V. Alveolar macrophage activation is a key initiation signal for acute lung ischemia-reperfusion injury. American journal of physiology. Lung cellular and molecular physiology. 2006;291(5): L1018-26. PMID: 16861385
- Ross S, Kron I, Gangemi J, Shockey K, Stoler M, Kern J, Tribble C, Laubach V. Attenuation of lung reperfusion injury after transplantation using an inhibitor of nuclear factor-kappaB. American journal of physiology. Lung cellular and molecular physiology. 2000;279(3): L528-36. PMID: 10956628