Richard J. Price

Education

  • BS, Rochester Institute of Technology
  • PhD, University of Virginia
  • Postdoc, University of Virginia
  • MS, University of Virginia
  • PhD, University of Virginia
  • BS, Rochester Institute of Technology
  • MS, University of Virginia

Primary Appointment

  • Associate Professor, Biomedical Engineering

Contact

Research Interest(s)

Endothelial Phenotypes in Angiogenesis, Integrated Signaling for Microvascular Network Patterning, and Contrast Ultrasound Based Therapeutics

Research Description

The formation of new microvessel networks is a critically important event in many normal and pathological adaptations. Proper network assembly involves formation of new capillaries then investment of these new capillaries with perivascular or smooth muscle cells. Our research seeks to understand how molecular signals and hemodynamic stresses coordinate the assembly of these networks. Specifically, the laboratory is studying how migrating vascular cells are guided by underlying matrix protein scaffolds and how the process of capillary investment with perivascular cells is controlled by signaling molecules produced by capillary wall cells in response to alterations in blood pressure and flow.

The delivery of genes and drugs encased in liposomes to specific regions of the body is often hindered by the endothelial lining of microvessels. We have observed that under certain conditions, insonified albumin-shelled microbubbles can produce ruptures in small skeletal muscle microvessels, allowing intravascular gene- and drug-bearing liposomes to pass across the endothelial lining in ultrasonically targeted regions in the body. Studies are currently aimed at determining optimal ultrasound parameters and microbubble composition for producing various rupture sizes in tissue and testing this technique for its ability to enhance the delivery of gene- and drug-bearing liposomes to tumors and myocardium.

Selected Publications

  • Nance E, Timbie K, Miller G, Song J, Louttit C, Klibanov A, Shih T, Swaminathan G, Tamargo R, Woodworth G, Hanes J, Price R. Non-invasive delivery of stealth, brain-penetrating nanoparticles across the blood-brain barrier using MRI-guided focused ultrasound. Journal of controlled release : official journal of the Controlled Release Society. 2014;189 123-32. PMID: 24979210 | PMCID: PMC4125545
  • Burke C, Alexander E, Timbie K, Kilbanov A, Price R. Ultrasound-activated agents comprised of 5FU-bearing nanoparticles bonded to microbubbles inhibit solid tumor growth and improve survival. Molecular therapy : the journal of the American Society of Gene Therapy. 2013;22(2): 321-8. PMID: 24172867 | PMCID: PMC3916048
  • Burke C, Suk J, Kim A, Hsiang Y, Klibanov A, Hanes J, Price R. Markedly enhanced skeletal muscle transfection achieved by the ultrasound-targeted delivery of non-viral gene nanocarriers with microbubbles. Journal of controlled release : official journal of the Controlled Release Society. 2012;162(2): 414-21. PMID: 22800583 | PMCID: PMC3438364
  • Meisner J, Song J, Price R. Arteriolar and Venular Remodeling Are Differentially Regulated by Bone Marrow-Derived Cell-Specific CX3CR1 and CCR2 Expression. PloS one. 2012;7(9): e46312. PMID: 23029475 | PMCID: PMC3454326
  • Meisner J, Sumer S, Murrell K, Higgins T, Price R. Laser speckle flowmetry method for measuring spatial and temporal hemodynamic alterations throughout large microvascular networks. Microcirculation (New York, N.Y. : 1994). 2012;19(7): 619-31. PMID: 22591575 | PMCID: PMC3434303
  • Burke C, Hsiang Y, Alexander E, Kilbanov A, Price R. Covalently linking poly(lactic-co-glycolic acid) nanoparticles to microbubbles before intravenous injection improves their ultrasound-targeted delivery to skeletal muscle. Small (Weinheim an der Bergstrasse, Germany). 2011;7(9): 1227-35. PMID: 21456081 | PMCID: PMC3092637
  • Burke C, Klibanov A, Sheehan J, Price R. Inhibition of glioma growth by microbubble activation in a subcutaneous model using low duty cycle ultrasound without significant heating. Journal of neurosurgery. 2011;114(6): 1654-61. PMID: 21214331
  • Burke C, Price R. Contrast ultrasound targeted treatment of gliomas in mice via drug-bearing nanoparticle delivery and microvascular ablation. Journal of visualized experiments : JoVE. 2011;. PMID: 21206463
  • Meisner J, Price R. Spatial and temporal coordination of bone marrow-derived cell activity during arteriogenesis: regulation of the endogenous response and therapeutic implications. Microcirculation (New York, N.Y. : 1994). 2010;17(8): 583-99. PMID: 21044213 | PMCID: PMC2974339
  • Nickerson M, Song J, Meisner J, Bajikar S, Burke C, Shuptrine C, Owens G, Skalak T, Price R. Bone marrow-derived cell-specific chemokine (C-C motif) receptor-2 expression is required for arteriolar remodeling. Arteriosclerosis, thrombosis, and vascular biology. 2009;29(11): 1794-801. PMID: 19734197 | PMCID: PMC2766019
  • Anderson C, Hastings N, Blackman B, Price R. Capillary sprout endothelial cells exhibit a CD36 low phenotype: regulation by shear stress and vascular endothelial growth factor-induced mechanism for attenuating anti-proliferative thrombospondin-1 signaling. The American journal of pathology. 2008;173(4): 1220-8. PMID: 18772338 | PMCID: PMC2543088
  • Song J, Klibanov A, Hossack J, Price R. Acoustic attenuation by contrast agent microbubbles in superficial tissue markedly diminishes petechiae bioeffects in deep tissue. Investigative radiology. 2008;43(5): 322-9. PMID: 18424953 | PMCID: PMC2714264
  • Wieghaus K, Nickerson M, Petrie Aronin C, Sefcik L, Price R, Paige M, Brown M, Botchwey E. Expansion of microvascular networks in vivo by phthalimide neovascular factor 1 (PNF1). Biomaterials. 2008;29(35): 4698-708. PMID: 18804278 | PMCID: PMC2885000
  • Chappell J, Price R. Targeted therapeutic applications of acoustically active microspheres in the microcirculation. Microcirculation (New York, N.Y. : 1994). 2006;13(1): 57-70. PMID: 16393947
  • Wieghaus K, Capitosti S, Anderson C, Price R, Blackman B, Brown M, Botchwey E. Small molecule inducers of angiogenesis for tissue engineering. Tissue engineering. 2006;12(7): 1903-13. PMID: 16889520