Ming D. Li

Education

  • PhD, University of Minnesota, Minneapolis, Mn
  • Postdoc, RLH U. S. Meat Animal Res Center, USDA-ARS
  • BS, Shanxi Agricultural University, Shanxi, PR China
  • MS, China Agricultural University, Beijing, PR China
  • MS, University of Minnesota, Minneapolis, Mn
  • Postdoc, University of Minnesota, School of Medicine

Primary Appointment

  • Professor, Psychiatry and Neurobehavioral Sciences

Contact

Research Interest(s)

Human Genetics and Genomics, Genetics Epidemiology and Bioinformatics, Molecular Neuroscience of Drug Addiction

Research Description



The primary research interest in Dr. Li’s laboratory is to understand how tobacco smokers become addicted and why they cannot quit their smoking habit although they realize that tobacco smoking is harmful to their health.Mounting evidence suggests that certain aspects of smoking are influenced by genetic factors. However, the genetic factors responsible for tobacco dependence are largely unknown. One of the NIH-funded research projects in Dr. Li’s laboratory is to identify susceptibility loci and genes for nicotine dependence using genome-wide linkage and association analysis approaches. Since 1999, more then 2,000 subjects representing approximately 600 smoker families of different ethnical populations have been recruited in multiple states of the U.S. and are being used for genetic analysis. Currently, we are concentrating on identification and characterization of hundreds of candidate genes selected from the positive regions identified by linkage analyses.

Another NIH-funded project in Dr. Li’s laboratory is employing microchip and proteomic technologies to identify novel genes and biochemical pathways associated with nicotine dependence and nicotine withdrawal in experimental animals. Several versions of custom-designed microchips have been developed in the laboratory and are being used in a number of microarray projects. Dr. Li’s laboratory is also using conventional molecular techniques such as Northern, RNase protection, real-time RT-PCR, in situ hybridization, Western, radioimmunoassay, radioligand binding assay, and immunohistochemistry in these projects and to investigate why nicotine suppresses appetite and increases energy expenditure of smokers.

Additionally, Dr. Li’s laboratory is interested in Bioinformatics. To accomplish the goals of genetic mapping, cDNA microchip and other ongoing projects, a Bioinformatics research group has been formed in the laboratory. The scientists of this group work closely with the batch scientists in the laboratory and other institutions. The primary research areas of interest in our Bioinformatics effort include linkage and association analyses, microarray data mining and analysis, protein modeling and molecular evolutionary analysis.

The research in this laboratory is currently being carried out by a dozen of research staff at various levels including Research faculty, Scientists, Post-Doctoral Fellows, Research Associates and Research Assistants, with very different scientific disciplines such as Psychology, Pharmacology, Neurosciences, Molecular Biology, Genetics, Computer Sciences, Bioinformatics, or Biostatistics.

Selected Publications

  • Cao J, Gautier N, Li M. CD-1 mice Show Individual Differences in Nicotine Preference in a Modified Two-Bottle Oral Self-Administration Model. Frontiers in psychiatry / Frontiers Research Foundation. 2012;3 28. PMID: 22479254 | PMCID: PMC3315826
  • Cui W, Zhao S, Polanowska-Grabowska R, Wang J, Wei J, Dash B, Chang S, Saucerman J, Gu J, Li M. Identification and Characterization of Poly(I:C)-induced Molecular Responses Attenuated by Nicotine in Mouse Macrophages. Molecular pharmacology. 2012. PMID: 23028093
  • Ma J, Li M, Payne T. Evaluation of the brief wisconsin inventory of smoking dependence motives in african-american and European-american heavy smokers. Frontiers in psychiatry / Frontiers Research Foundation. 2012;3 36. PMID: 22557980 | PMCID: PMC3337480
  • Chen J, Brunzell D, Jackson K, van der Vaart A, Ma J, Payne T, Sherva R, Farrer L, Gejman P, Levinson D, Holmans P, Aggen S, Damaj I, Kuo P, Webb B, Anton R, Kranzler H, Gelernter J, Li M, Kendler K, Chen X. ACSL6 is associated with the number of cigarettes smoked and its expression is altered by chronic nicotine exposure. PloS one. 2011;6(12): e28790. PMID: 22205969 | PMCID: PMC3243669
  • Johnson B, Ait-Daoud N, Seneviratne C, Roache J, Javors M, Wang X, Liu L, Penberthy J, DiClemente C, Li M. Pharmacogenetic approach at the serotonin transporter gene as a method of reducing the severity of alcohol drinking. The American journal of psychiatry. 2011;168(3): 265-75. PMID: 21247998 | PMCID: PMC3063997
  • Li M, van der Vaart A. MicroRNAs in addiction: adaptation's middlemen? Molecular psychiatry. 2011;16(12): 1159-68. PMID: 21606928
  • Wang J, Cui W, Wei J, Sun D, Gutala R, Gu J, Li M. Genome-wide expression analysis reveals diverse effects of acute nicotine exposure on neuronal function-related genes and pathways. Frontiers in psychiatry / Frontiers Research Foundation. 2011;2 5. PMID: 21556275 | PMCID: PMC3089989
  • Wang J, Yuan W, Li M. Genes and pathways co-associated with the exposure to multiple drugs of abuse, including alcohol, amphetamine/methamphetamine, cocaine, marijuana, morphine, and/or nicotine: a review of proteomics analyses. Molecular neurobiology. 2011;44(3): 269-86. PMID: 21922273
  • Identifying susceptibility loci for nicotine dependence: 2008 update based on recent genome-wide linkage analyses. Hum Genet. 2008;123(2): 119-31.
  • Huang W, Kane J, Li M. Identification and characterization of a long isoform of human IFT80, IFT80-L. Biochem Biophys Res Commun. 2008;373(4): 653-8.
  • Huang W, Ma J, Payne T, Beuten J, Dupont R, Li M. Significant association of DRD1 with nicotine dependence. Hum Genet. 2008;123(2): 133-40.
  • Huang W, Payne T, Ma J, Li M. A functional polymorphism, rs6280, in DRD3 is significantly associated with nicotine dependence in European-American smokers. Am J Med Genet B Neuropsychiatr Genet. 2008;147(7): 1109-15.
  • Li M, Lou X, Chen G, Ma J, Elston R. Gene-gene interactions among CHRNA4, CHRNB2, BDNF, and NTRK2 in nicotine dependence. Biol Psychiatry. 2008;64(11): 951-7. PMCID: PMC2592606
  • Li M, Ma J, Payne T, Lou X, Zhang D, Dupont R, Elston R. Genome-wide linkage scan for nicotine dependence in European Americans and its converging results with African Americans in the Mid-South Tobacco Family sample. Mol Psychiatry. 2008;13(4): 407-16.
  • Li M, Stukenberg P, Brautigan D. Binding of phosphatase inhibitor-2 to prolyl isomerase Pin1 modifies specificity for mitotic phosphoproteins. Biochemistry. 2008;47(1): 292-300.
  • Lou X, Chen G, Yan L, Ma J, Mangold J, Zhu J, Elston R, Li M. A combinatorial approach to detecting gene-gene and gene-environment interactions in family studies. Am J Hum Genet. 2008;83(4): 457-67. PMCID: PMC2561932
  • Mangold J, Payne T, Ma J, Chen G, Li M. Bitter taste receptor gene polymorphisms are an important factor in the development of nicotine dependence in African Americans. J Med Genet. 2008;45(9): 578-82.
  • Nussbaum J, Xu Q, Payne T, Ma J, Huang W, Gelernter J, Li M. Significant association of the neurexin-1 gene (NRXN1) with nicotine dependence in European- and African-American smokers. Hum Mol Genet. 2008;17(11): 1569-77. PMCID: PMC2902291
  • Sun D, Ma J, Payne T, Li M. Beta-arrestins 1 and 2 are associated with nicotine dependence in European American smokers. Mol Psychiatry. 2008;13(4): 398-406.
  • Wang J, Gutala R, Hwang Y, Kim J, Konu O, Ma J, Li M. Strain- and region-specific gene expression profiles in mouse brain in response to chronic nicotine treatment. Genes Brain Behav. 2008;7(1): 78-87.
  • The genetics of nicotine dependence. Curr Psychiatry Rep. 8(2): 158-64.
  • Beuten J, Ma J, Lou X, Payne T, Li M. Association analysis of the protein phosphatase 1 regulatory subunit 1B (PPP1R1B) gene with nicotine dependence in European- and African-American smokers. Am J Med Genet B Neuropsychiatr Genet. 144(3): 285-90.
  • Beuten J, Ma J, Payne T, Dupont R, Lou X, Crews K, Elston R, Li M. Association of specific haplotypes of neurotrophic tyrosine kinase receptor 2 gene (NTRK2) with vulnerability to nicotine dependence in African-Americans and European-Americans. Biol Psychiatry. 61(1): 48-55.
  • Cao J, Dwyer J, Mangold J, Wang J, Wei J, Leslie F, Li M. Modulation of cell adhesion systems by prenatal nicotine exposure in limbic brain regions of adolescent female rats. Int J Neuropsychopharmacol. 1-18.
  • Cui W, Li M. Erratum to: Nicotinic Modulation of Innate Immune Pathways Via alpha7 Nicotinic Acetylcholine Receptor. J Neuroimmune Pharmacol. .
  • Cui W, Li M. Nicotinic Modulation of Innate Immune Pathways Via alpha7 Nicotinic Acetylcholine Receptor. J Neuroimmune Pharmacol. .
  • Glesner J, Wünschmann S, Li M, Gustchina A, Wlodawer A, Himly M, Chapman M, Pomés A. Mechanisms of Allergen-Antibody Interaction of Cockroach Allergen Bla g 2 with Monoclonal Antibodies That Inhibit IgE Antibody Binding. PLoS One. 6(7): e22223. PMCID: PMC3137622
  • Gutala R, Wang J, Hwang Y, Haq R, Li M. Nicotine modulates expression of amyloid precursor protein and amyloid precursor-like protein 2 in mouse brain and in SH-SY5Y neuroblastoma cells. Brain Res. 1093(1): 12-19.
  • Huang W, Li M. Differential allelic expression of dopamine D1 receptor gene (DRD1) is modulated by microRNA miR-504. Biol Psychiatry. 65(8): 702-5. PMCID: PMC2678413
  • Huang W, Li M. Nicotine modulates expression of miR-140*, which targets the 3'-untranslated region of dynamin 1 gene (Dnm1). Int J Neuropsychopharmacol. 12(4): 537-46.
  • Huang W, Payne T, Ma J, Beuten J, Dupont R, Inohara N, Li M. Significant association of ANKK1 and detection of a functional polymorphism with nicotine dependence in an African-American sample. Neuropsychopharmacology. 34(2): 319-30.
  • Hwang Y, Li M. Proteins differentially expressed in response to nicotine in five rat brain regions: identification using a 2-DE/MS-based proteomics approach. Proteomics. 6(10): 3138-53.
  • Johnson B, Ait-Daoud N, Seneviratne C, Roache J, Javors M, Wang X, Liu L, Penberthy J, DiClemente C, Li M. Pharmacogenetic approach at the serotonin transporter gene as a method of reducing the severity of alcohol drinking. Am J Psychiatry. 168(3): 265-75. PMCID: PMC3063997
  • Li M, Burmeister M. New insights into the genetics of addiction. Nat Rev Genet. 10(4): 225-31. PMCID: PMC2879628
  • Li M, Mangold J, Seneviratne C, Chen G, Ma J, Lou X, Payne T. Association and interaction analyses of GABBR1 and GABBR2 with nicotine dependence in European- and African-American populations. PLoS One. 4(9): e7055. PMCID: PMC2739294
  • Li M, Payne T, Ma J, Lou X, Zhang D, Dupont R, Crews K, Somes G, Williams N, Elston R. A genomewide search finds major susceptibility loci for nicotine dependence on chromosome 10 in African Americans. Am J Hum Genet. 79(4): 745-51. PMCID: PMC1592559
  • Li M, Satinover D, Brautigan D. Phosphorylation and functions of inhibitor-2 family of proteins. Biochemistry. 46(9): 2380-9.
  • Li M, Sun D, Lou X, Beuten J, Payne T, Ma J. Linkage and association studies in African- and Caucasian-American populations demonstrate that SHC3 is a novel susceptibility locus for nicotine dependence. Mol Psychiatry. 12(5): 462-73.
  • Li M, Xu Q, Lou X, Payne T, Niu T, Ma J. Association and interaction analysis of variants in CHRNA5/CHRNA3/CHRNB4 gene cluster with nicotine dependence in African and European Americans. Am J Med Genet B Neuropsychiatr Genet. 153(3): 745-56. PMCID: PMC2924635
  • Li M, van A. MicroRNAs in addiction: adaptation's middlemen? Mol Psychiatry. .
  • Lou X, Chen G, Yan L, Ma J, Zhu J, Elston R, Li M. A generalized combinatorial approach for detecting gene-by-gene and gene-by-environment interactions with application to nicotine dependence. Am J Hum Genet. 80(6): 1125-37. PMCID: PMC1867100
  • Lou X, Ma J, Payne T, Beuten J, Crew K, Li M. Gene-based analysis suggests association of the nicotinic acetylcholine receptor beta1 subunit (CHRNB1) and M1 muscarinic acetylcholine receptor (CHRM1) with vulnerability for nicotine dependence. Hum Genet. 120(3): 381-9.
  • Lou X, Ma J, Sun D, Payne T, Li M. Fine mapping of a linkage region on chromosome 17p13 reveals that GABARAP and DLG4 are associated with vulnerability to nicotine dependence in European-Americans. Hum Mol Genet. 16(2): 142-53.
  • Ma J, Payne T, Li M. Significant association of glutamate receptor, ionotropic N-methyl-D-aspartate 3A (GRIN3A), with nicotine dependence in European- and African-American smokers. Hum Genet. 127(5): 503-12.
  • Seneviratne C, Ait-Daoud N, Ma J, Chen G, Johnson B, Li M. Susceptibility locus in neurokinin-1 receptor gene associated with alcohol dependence. Neuropsychopharmacology. 34(11): 2442-9. PMCID: PMC2743763
  • Seneviratne C, Huang W, Ait-Daoud N, Li M, Johnson B. Characterization of a functional polymorphism in the 3' UTR of SLC6A4 and its association with drinking intensity. Alcohol Clin Exp Res. 33(2): 332-9. PMCID: PMC2657929
  • Sun D, Huang W, Hwang Y, Zhang Y, Zhang Q, Li M. Regulation by nicotine of Gpr51 and Ntrk2 expression in various rat brain regions. Neuropsychopharmacology. 32(1): 110-6.
  • Wang J, Cui W, Wei J, Sun D, Gutala R, Gu J, Li M. Genome-wide expression analysis reveals diverse effects of acute nicotine exposure on neuronal function-related genes and pathways. Front Psychiatry. 2 5.0. PMCID: PMC3089989
  • Wang J, Gutala R, Sun D, Ma J, Sheela R, Ticku M, Li M. Regulation of platelet-derived growth factor signaling pathway by ethanol, nicotine, or both in mouse cortical neurons. Alcohol Clin Exp Res. 31(3): 357-75.
  • Wang J, Kim J, Donovan D, Becker K, Li M. Significant modulation of mitochondrial electron transport system by nicotine in various rat brain regions. Mitochondrion. 9(3): 186-95. PMCID: PMC2783500
  • Wang J, Li M. Common and unique biological pathways associated with smoking initiation/progression, nicotine dependence, and smoking cessation. Neuropsychopharmacology. 35(3): 702-19. PMCID: PMC2814000
  • Wei J, Ma J, Payne T, Cui W, Ray R, Mitra N, Lerman C, Li M. Replication and extension of association of choline acetyltransferase with nicotine dependence in European and African American smokers. Hum Genet. 127(6): 691-8.
  • Wei J, Wang J, Dwyer J, Mangold J, Cao J, Leslie F, Li M. Gestational nicotine treatment modulates cell death/survival-related pathways in the brains of adolescent female rats. Int J Neuropsychopharmacol. 1-16.
  • Xu Q, Huang W, Payne T, Ma J, Li M. Detection of genetic association and a functional polymorphism of dynamin 1 gene with nicotine dependence in European and African Americans. Neuropsychopharmacology. 34(5): 1351-9. PMCID: PMC2656577