Matthew J Wolf


  • MD, Washington University School of Medicine
  • PhD, Washington University School of Medicine
  • Residency, Duke University School of Medicine, Durham, NC
  • Fellowship, Duke University School of Medicine, Durham, NC

Primary Appointment

  • Associate Professor, Medicine- Cardiovascular Medicine


Research Interest(s)

Identification of genes and pathways that cause or modify cardiac hypertrophy and heart failure.

Research Description

Our mission is to understand how signaling molecules mediate cardiomyocyte growth, hypertrophy, and heart failure. We use novel approaches based on fruit fly genetics to identify genes that cause or modify cardiomyopathies and translate these findings using mammalian cell-based systems and transgenic mice. Our multidisciplinary approach employs state-of-the-art Optical Coherence Tomography, confocal microscopy, molecular and cell biology, proteomics, and physiology to understand cardiovascular disease.

Selected Publications

  • Wolf M, Noeth D, Rammohan C, Shah S. Complexities of Genetic Testing in Familial Dilated Cardiomyopathy. Circulation. Cardiovascular genetics. 2016;9(1): 95-9. PMID: 26884609
  • Gao Q, Wyatt E, Goldstein J, LoPresti P, Castillo L, Gazda A, Petrossian N, Earley J, Hadhazy M, Barefield D, Demonbreun A, Bönnemann C, Wolf M, McNally E. Reengineering a transmembrane protein to treat muscular dystrophy using exon skipping. The Journal of clinical investigation. 2015;125(11): 4186-95. PMID: 26457733 | PMCID: PMC4639981
  • Hardy C, Birse R, Wolf M, Yu L, Bodmer R, Gibbs A. Obesity-associated cardiac dysfunction in starvation-selected Drosophila melanogaster. American journal of physiology. Regulatory, integrative and comparative physiology. 2015;309(6): R658-67. PMID: 26136533 | PMCID: PMC4591367
  • Yu L, Daniels J, Wu H, Wolf M. Cardiac hypertrophy induced by active Raf depends on Yorkie-mediated transcription. Science signaling. 2015;8(362): ra13. PMID: 25650441
  • Towards understanding the impact of sarcomeric gene mutations. JACC. Heart failure. 2014;1(6): 467-8. PMID: 24621998
  • Lee T, Yu L, Wolf M, Rockman H. Galactokinase is a novel modifier of calcineurin-induced cardiomyopathy in Drosophila. Genetics. 2014;198(2): 591-603. PMID: 25081566 | PMCID: PMC4196615
  • Abraham D, Wolf M. Disruption of sarcoendoplasmic reticulum calcium ATPase function in Drosophila leads to cardiac dysfunction. PloS one. 2013;8(10): e77785. PMID: 24098595 | PMCID: PMC3789689
  • Yu L, Daniels J, Glaser A, Wolf M. Raf-mediated cardiac hypertrophy in adult Drosophila. Disease models & mechanisms. 2013;6(4): 964-76. PMID: 23580199 | PMCID: PMC3701216
  • Modeling dilated cardiomyopathies in Drosophila. Trends in cardiovascular medicine. 2012;22(3): 55-61. PMID: 22863366 | PMCID: PMC3728662
  • Casad M, Yu L, Daniels J, Wolf M, Rockman H. Deletion of Siah-interacting protein gene in Drosophila causes cardiomyopathy. Molecular genetics and genomics : MGG. 2012;287(4): 351-60. PMID: 22398840 | PMCID: PMC3701947
  • Wooten E, Hebl V, Wolf M, Greytak S, Orr N, Draper I, Calvino J, Kapur N, Maron M, Kullo I, Ommen S, Bos J, Ackerman M, Huggins G. Formin homology 2 domain containing 3 variants associated with hypertrophic cardiomyopathy. Circulation. Cardiovascular genetics. 2012;6(1): 10-8. PMID: 23255317 | PMCID: PMC3578062
  • Casad M, Abraham D, Kim I, Frangakis S, Dong B, Lin N, Wolf M, Rockman H. Cardiomyopathy is associated with ribosomal protein gene haplo-insufficiency in Drosophila melanogaster. Genetics. 2011;189(3): 861-70. PMID: 21890737 | PMCID: PMC3213377
  • Lin N, Badie N, Yu L, Abraham D, Cheng H, Bursac N, Rockman H, Wolf M. A method to measure myocardial calcium handling in adult Drosophila. Circulation research. 2011;108(11): 1306-15. PMID: 21493892 | PMCID: PMC3128985
  • Wolf M, Rockman H. Drosophila, genetic screens, and cardiac function. Circulation research. 2011;109(7): 794-806. PMID: 21921272 | PMCID: PMC3678974
  • Goldstein J, Kelly S, LoPresti P, Heydemann A, Earley J, Ferguson E, Wolf M, McNally E. SMAD signaling drives heart and muscle dysfunction in a Drosophila model of muscular dystrophy. Human molecular genetics. 2010;20(5): 894-904. PMID: 21138941 | PMCID: PMC3033181
  • Kim I, Wolf M, Rockman H. Gene deletion screen for cardiomyopathy in adult Drosophila identifies a new notch ligand. Circulation research. 2010;106(7): 1233-43. PMID: 20203305 | PMCID: PMC2860286
  • Yu L, Lee T, Lin N, Wolf M. Affecting Rhomboid-3 function causes a dilated heart in adult Drosophila. PLoS genetics. 2010;6(5): e1000969. PMID: 20523889 | PMCID: PMC2877733
  • Kim I, Wolf M. Serial examination of an inducible and reversible dilated cardiomyopathy in individual adult Drosophila. PloS one. 2009;4(9): e7132. PMID: 19771157 | PMCID: PMC2740824
  • Wolf M, Rockman H. Drosophila melanogaster as a model system for genetics of postnatal cardiac function. Drug discovery today. Disease models. 2009;5(3): 117-123. PMID: 19802348 | PMCID: PMC2753975
  • Yoo B, Lemaire A, Mangmool S, Wolf M, Curcio A, Mao L, Rockman H. Beta1-adrenergic receptors stimulate cardiac contractility and CaMKII activation in vivo and enhance cardiac dysfunction following myocardial infarction. American journal of physiology. Heart and circulatory physiology. 2009;297(4): H1377-86. PMID: 19633206 | PMCID: PMC2770777
  • Allikian M, Bhabha G, Dospoy P, Heydemann A, Ryder P, Earley J, Wolf M, Rockman H, McNally E. Reduced life span with heart and muscle dysfunction in Drosophila sarcoglycan mutants. Human molecular genetics. 2007;16(23): 2933-43. PMID: 17855453
  • Curcio A, Noma T, Naga Prasad S, Wolf M, Lemaire A, Perrino C, Mao L, Rockman H. Competitive displacement of phosphoinositide 3-kinase from beta-adrenergic receptor kinase-1 improves postinfarction adverse myocardial remodeling. American journal of physiology. Heart and circulatory physiology. 2006;291(4): H1754-60. PMID: 16699071
  • Volovyk Z, Wolf M, Prasad S, Rockman H. Agonist-stimulated beta-adrenergic receptor internalization requires dynamic cytoskeletal actin turnover. The Journal of biological chemistry. 2006;281(14): 9773-80. PMID: 16461348
  • Wolf M, Amrein H, Izatt J, Choma M, Reedy M, Rockman H. Drosophila as a model for the identification of genes causing adult human heart disease. Proceedings of the National Academy of Sciences of the United States of America. 2006;103(5): 1394-9. PMID: 16432241 | PMCID: PMC1360529
  • Perrino C, Naga Prasad S, Patel M, Wolf M, Rockman H. Targeted inhibition of beta-adrenergic receptor kinase-1-associated phosphoinositide-3 kinase activity preserves beta-adrenergic receptor signaling and prolongs survival in heart failure induced by calsequestrin overexpression. Journal of the American College of Cardiology. 2005;45(11): 1862-70. PMID: 15936620
  • Wheeler F, Fernandez L, Carlson K, Wolf M, Rockman H, Marchuk D. QTL mapping in a mouse model of cardiomyopathy reveals an ancestral modifier allele affecting heart function and survival. Mammalian genome : official journal of the International Mammalian Genome Society. 2005;16(6): 414-23. PMID: 16075368
  • Wolf M, Tachibana H, Rockman H. Methods for the detection of altered beta-adrenergic receptor signaling pathways in hypertrophied hearts. Methods in molecular medicine. 2005;112 353-62. PMID: 16010029
  • Jenkins C, Wolf M, Mancuso D, Gross R. Identification of the calmodulin-binding domain of recombinant calcium-independent phospholipase A2beta. implications for structure and function. The Journal of biological chemistry. 2000;276(10): 7129-35. PMID: 11118454
  • Ramanadham S, Wolf M, Li B, Bohrer A, Turk J. Glucose-responsitivity and expression of an ATP-stimulatable, Ca(2+)-independent phospholipase A2 enzyme in clonal insulinoma cell lines. Biochimica et biophysica acta. 1997;1344(2): 153-64. PMID: 9030192
  • Wolf M, Wang J, Turk J, Gross R. Depletion of intracellular calcium stores activates smooth muscle cell calcium-independent phospholipase A2. A novel mechanism underlying arachidonic acid mobilization. The Journal of biological chemistry. 1997;272(3): 1522-6. PMID: 8999823
  • Ramanadham S, Wolf M, Ma Z, Li B, Wang J, Gross R, Turk J. Evidence for association of an ATP-stimulatable Ca(2+)-independent phospholipase A2 from pancreatic islets and HIT insulinoma cells with a phosphofructokinase-like protein. Biochemistry. 1996;35(17): 5464-71. PMID: 8611537
  • Wolf M, Gross R. Expression, purification, and kinetic characterization of a recombinant 80-kDa intracellular calcium-independent phospholipase A2. The Journal of biological chemistry. 1996;271(48): 30879-85. PMID: 8940072
  • Wolf M, Gross R. The calcium-dependent association and functional coupling of calmodulin with myocardial phospholipase A2. Implications for cardiac cycle-dependent alterations in phospholipolysis. The Journal of biological chemistry. 1996;271(35): 20989-92. PMID: 8702861
  • Gross R, Rudolph A, Wang J, Sommers C, Wolf M. Nitric oxide activates the glucose-dependent mobilization of arachidonic acid in a macrophage-like cell line (RAW 264.7) that is largely mediated by calcium-independent phospholipase A2. The Journal of biological chemistry. 1995;270(25): 14855-8. PMID: 7797462
  • Wolf M, Izumi Y, Zorumski C, Gross R. Long-term potentiation requires activation of calcium-independent phospholipase A2. FEBS letters. 1995;377(3): 358-62. PMID: 8549755
  • Hazen S, Wolf M, Ford D, Gross R. The rapid and reversible association of phosphofructokinase with myocardial membranes during myocardial ischemia. FEBS letters. 1994;339(3): 213-6. PMID: 8112458
  • Ramanadham S, Wolf M, Jett P, Gross R, Turk J. Characterization of an ATP-stimulatable Ca(2+)-independent phospholipase A2 from clonal insulin-secreting HIT cells and rat pancreatic islets: a possible molecular component of the beta-cell fuel sensor. Biochemistry. 1994;33(23): 7442-52. PMID: 8003509
  • Molecular Cardiology Humana Press. 353-362.
  • Ardehali Hossein, Bolli Roberto, Losordo Douglas. REsearch Techiques in Cardiovascular Medicine Wiley Blackwell. 432-439.