- BS, University of Tubingen
- PhD, Free University of Berlin
- Postdoc, Oregon Health Sciences University
- Assistant Professor, Neuroscience
- Email: email@example.com
- Website: http://www.healthsystem.virginia.edu/internet/neurosci/Faculty/Shin/home.cfm
Hearing loss is America’s leading disability, affecting millions of people of all ages. To develop preventative and restorative clinical approaches, it is crucial to understand how the hearing process works on the cellular and molecular level. Hearing is mediated by specialized hair cells in the inner ear, and the goal of our lab is to learn more about their function: how do hair cells develop, what proteins are they made of, and what are the molecular mechanisms of their dysfunction that cause hearing loss and balance defects? To this end, we use a variety of techniques to identify and characterize proteins involved in hair cell function.
Our lab is especially interested in the question of how the hair cell maintains its integrity over the years. Hair cells are among the cell types that cannot be renewed and therefore require a special maintenance strategy. Our hypothesis is that hair cells maintain their functionality by constantly turning over its proteins, and we use metabolic labeling in combination with peptide mass spectrometry to measure protein turnover in hair cells. We are especially interested in changes that occur during pathological stress situations (mechanical and oxidative stress).
Another focus of our lab is the dynamic behavior of hair cell proteins, especially components involved in the mechanotransduction process. We want to describe the subcellular movements of proteins that are involved in establishing and executing the mechanotransduction process, using in-vivo imaging. We will generate transgenic Xenopus frogs that express GFP-tagged proteins in hair cells and monitor the movement of these proteins in the living hair cell. We are especially interested in observing the dynamic processes associated with the disruption of the transduction complex caused by cellular stress situations, such as oxidative and mechanical stress.
- Shin J, Adams D, Paukert M, Siba M, Sidi S, Levin M, Gillespie P, Gründer S. Xenopus TRPN1 (NOMPC) localizes to microtubule-based cilia in epithelial cells, including inner-ear hair cells. Proceedings of the National Academy of Sciences of the United States of America. 2005;102(35): 12572-7. PMID: 16116094 | PMCID: PMC1194908
- Senften M, Schwander M, Kazmierczak P, Lillo C, Shin J, Hasson T, Géléoc G, Gillespie P, Williams D, Holt J, Müller U. Physical and functional interaction between protocadherin 15 and myosin VIIa in mechanosensory hair cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2006;26(7): 2060-71. PMID: 16481439 | PMCID: PMC2712835
- Gagnon L, Longo-Guess C, Berryman M, Shin J, Saylor K, Yu H, Gillespie P, Johnson K. The chloride intracellular channel protein CLIC5 is expressed at high levels in hair cell stereocilia and is essential for normal inner ear function. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2006;26(40): 10188-98. PMID: 17021174
- Shin J, Streijger F, Beynon A, Peters T, Gadzala L, McMillen D, Bystrom C, Van der Zee C, Wallimann T, Gillespie P. Hair bundles are specialized for ATP delivery via creatine kinase. Neuron. 2007;53(3): 371-86. PMID: 17270734 | PMCID: PMC1839076
- Shin J, Longo-Guess C, Gagnon L, Saylor K, Dumont R, Spinelli K, Pagana J, Wilmarth P, David L, Gillespie P, Johnson K. The R109H variant of fascin-2, a developmentally regulated actin crosslinker in hair-cell stereocilia, underlies early-onset hearing loss of DBA/2J mice. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2010;30(29): 9683-94. PMID: 20660251 | PMCID: PMC2922854
- Spinelli K, Klimek J, Wilmarth P, Shin J, Choi D, David L, Gillespie P. Distinct energy metabolism of auditory and vestibular sensory epithelia revealed by quantitative mass spectrometry using MS2 intensity. Proceedings of the National Academy of Sciences of the United States of America. 2012;109(5): E268-77. PMID: 22307652 | PMCID: PMC3277109
- Zhao H, Williams D, Shin J, Brügger B, Gillespie P. Large membrane domains in hair bundles specify spatially constricted radixin activation. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2012;32(13): 4600-9. PMID: 22457506 | PMCID: PMC3324267
- Grati M, Shin J, Weston M, Green J, Bhat M, Gillespie P, Kachar B. Localization of PDZD7 to the stereocilia ankle-link associates this scaffolding protein with the Usher syndrome protein network. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2012;32(41): 14288-93. PMID: 23055499 | PMCID: PMC3518401
- Shin J, Krey J, Hassan A, Metlagel Z, Tauscher A, Pagana J, Sherman N, Jeffery E, Spinelli K, Zhao H, Wilmarth P, Choi D, David L, Auer M, Barr-Gillespie P. Molecular architecture of the chick vestibular hair bundle. Nature neuroscience. 2013;16(3): 365-74. PMID: 23334578 | PMCID: PMC3581746
- Francis S, Katz J, Fanning K, Harris K, Nicholas B, Lacy M, Pagana J, Agris P, Shin J. A novel role of cytosolic protein synthesis inhibition in aminoglycoside ototoxicity. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2013;33(7): 3079-93. PMID: 23407963