John Campbell


  • BS, College of William and Mary
  • PhD, Virginia Commonwealth University School of Medicine


Research Interest(s)

The Neurobiology of Energy Balance

Research Description

The Campbell lab uses single-cell transcriptomics and genetic technology to identify the cell types and signaling pathways that control appetite, digestion, and glucose metabolism. Specifically, we apply high-throughput single-cell RNA-Seq to characterize cell types and their marker genes in specific brain regions and to generate hypotheses about the functions of those cell types. These marker genes enable genetic access to each cell type of interest, through the combined use of marker-driven recombinase-expressing mouse strains and targeted injections of recombinase-dependent AAV vectors. Using these AAVs and the transgenic tools they deliver, we map, monitor, and manipulate the activity of specific neuron populations, revealing their synaptic circuitry and physiological function. The ultimate goal of this approach is to match molecularly-defined cell types and circuits to their specific roles in appetite, digestion, and glucose metabolism.

Selected Publications

  • Campbell J, Macosko E, Fenselau H, Pers T, Lyubetskaya A, Tenen D, Goldman M, Verstegen A, Resch J, McCarroll S, Rosen E, Lowell B, Tsai L. A molecular census of arcuate hypothalamus and median eminence cell types. Nature neuroscience. 2017;20(3): 484-496. PMID: 28166221 | PMCID: PMC5323293
  • D'Agostino G, Lyons D, Cristiano C, Burke L, Madara J, Campbell J, Garcia A, Land B, Lowell B, Dileone R, Heisler L. Appetite controlled by a cholecystokinin nucleus of the solitary tract to hypothalamus neurocircuit. eLife. 2016;5. PMID: 26974347 | PMCID: PMC4861598
  • Fenselau H, Campbell J, Verstegen A, Madara J, Xu J, Shah B, Resch J, Yang Z, Mandelblat-Cerf Y, Livneh Y, Lowell B. A rapidly acting glutamatergic ARC→PVH satiety circuit postsynaptically regulated by α-MSH. Nature neuroscience. 2016;20(1): 42-51. PMID: 27869800 | PMCID: PMC5191921
  • Garfield A, Shah B, Burgess C, Li M, Li C, Steger J, Madara J, Campbell J, Kroeger D, Scammell T, Tannous B, Myers M, Andermann M, Krashes M, Lowell B. Dynamic GABAergic afferent modulation of AgRP neurons. Nature neuroscience. 2016;19(12): 1628-1635. PMID: 27643429 | PMCID: PMC5382799
  • Kong D, Dagon Y, Campbell J, Guo Y, Yang Z, Yi X, Aryal P, Wellenstein K, Kahn B, Sabatini B, Lowell B. A Postsynaptic AMPK→p21-Activated Kinase Pathway Drives Fasting-Induced Synaptic Plasticity in AgRP Neurons. Neuron. 2016;91(1): 25-33. PMID: 27321921 | PMCID: PMC4938763
  • Garfield A, Li C, Madara J, Shah B, Webber E, Steger J, Campbell J, Gavrilova O, Lee C, Olson D, Elmquist J, Tannous B, Krashes M, Lowell B. A neural basis for melanocortin-4 receptor-regulated appetite. Nature neuroscience. 2015;18(6): 863-71. PMID: 25915476 | PMCID: PMC4446192
  • Campbell J. N., Gandhi A., Singh B., Churn S. B.. Traumatic Brain Injury Causes a Tacrolimus-Sensitive Increase in Non-Convulsive Seizures in a Rat Model of Post-Traumatic Epilepsy Int J Neurol Brain Disord. 1(1): 1-11.
  • Campbell J. N., Low B., Kurz J. E., Patel S. S., Young M. T., Churn S. B.. Mechanisms of dendritic spine remodeling in a rat model of traumatic brain injury J Neurotrauma. 29(2): 218-34.
  • Campbell J. N., Register D., Churn S. B.. Traumatic brain injury causes an FK506-sensitive loss and an overgrowth of dendritic spines in rat forebrain J Neurotrauma. 29(2): 201-17.
  • Fenselau H., Campbell J. N., Verstegen A. M. J., Madara J. C., Xu J., Shah B. P., Resch J. M., Yang Z., Mandelblat-Cerf Y., Livneh Y., Lowell B. B.. Corrigendum: A rapidly acting glutamatergic ARC-->PVH satiety circuit postsynaptically regulated by alpha-MSH Nat Neurosci. 20(8): 1189.
  • Kurz J. E., Moore B. J., Henderson S. C., Campbell J. N., Churn S. B.. A cellular mechanism for dendritic spine loss in the pilocarpine model of status epilepticus Epilepsia. 49(10): 1696-710.
  • Li M. M., Madara J. C., Steger J. S., Krashes M. J., Balthasar N., Campbell J. N., Resch J. M., Conley N. J., Garfield A. S., Lowell B. B.. The Paraventricular Hypothalamus Regulates Satiety and Prevents Obesity via Two Genetically Distinct Circuits Neuron. .
  • Resch J. M., Fenselau H., Madara J. C., Wu C., Campbell J. N., Lyubetskaya A., Dawes B. A., Tsai L. T., Li M. M., Livneh Y., Ke Q., Kang P. M., Fejes-Toth G., Naray-Fejes-Toth A., Geerling J. C., Lowell B. B.. Aldosterone-Sensing Neurons in the NTS Exhibit State-Dependent Pacemaker Activity and Drive Sodium Appetite via Synergy with Angiotensin II Signaling Neuron. 96(1): 190-206 e7.