Joanna B. Goldberg

Education

  • BA, Boston University
  • PhD, University of California, Berkeley

Contact

Research Interest(s)

Mechanisms of Bacterial Pathogenesis

Research Description

The major focus of my laboratory is the investigation of strategies used by bacteria to cause diseases in humans. We study various bacteria and their factors especially surface polysaccharides and other potential adhesins and assess their effect on the virulence and physiology of the bacterium as well as on host cells. Our general approach is to construct and characterize bacterial mutants and monitor these for relevant phenotypic and genotypic characteristics and in in vivo and in vitro models of infection. In addition, we are constructing vaccines to these pathogens based on the expression of polysaccharides and testing these in mouse models of infection. The goal of these studies is to develop rational strategies to disrupt virulence and promote clearance of pathogenic bacteria.

Selected Publications

  • Damron F, Goldberg J. Proteolytic regulation of alginate overproduction in Pseudomonas aeruginosa. Molecular microbiology. 2012;84(4): 595-607. PMID: 22497280 | PMCID: PMC3345095
  • Damron F, Owings J, Okkotsu Y, Varga J, Schurr J, Goldberg J, Schurr M, Yu H. Analysis of the Pseudomonas aeruginosa regulon controlled by the sensor kinase KinB and sigma factor RpoN. Journal of bacteriology. 2012;194(6): 1317-30. PMID: 22210761 | PMCID: PMC3294845
  • Davis M, Goldberg J. Purification and visualization of lipopolysaccharide from Gram-negative bacteria by hot aqueous-phenol extraction. Journal of visualized experiments : JoVE. 2012;. PMID: 22688346
  • Liechti G, Goldberg J. Outer membrane biogenesis in Escherichia coli, Neisseria meningitidis, and Helicobacter pylori: paradigm deviations in H. pylori. Frontiers in cellular and infection microbiology. 2012;2 29. PMID: 22919621 | PMCID: PMC3417575
  • Porter L, Goldberg J. Influence of Neutrophil Defects on Burkholderia cepacia Complex Pathogenesis. Frontiers in cellular and infection microbiology. 2012;1 9. PMID: 22919575 | PMCID: PMC3417359
  • Varga J, Vigil A, Deshazer D, Waag D, Felgner P, Goldberg J. Distinct human antibody response to the biological warfare agent Burkholderia mallei. Virulence. 2012;3(6). PMID: 23076276
  • Goldberg J, Ganesan S, Comstock A, Zhao Y, Sajjan U. Cable pili and the associated 22 kDa adhesin contribute to Burkholderia cenocepacia persistence in vivo. PloS one. 2011;6(7): e22435. PMID: 21811611 | PMCID: PMC3141045
  • Kintz E, Goldberg J. Site-directed mutagenesis reveals key residue for O antigen chain length regulation and protein stability in Pseudomonas aeruginosa Wzz2. The Journal of biological chemistry. 2011;286(51): 44277-84. PMID: 22069314 | PMCID: PMC3243511
  • Liechti G, Goldberg J. Helicobacter pylori relies primarily on the purine salvage pathway for purine nucleotide biosynthesis. Journal of bacteriology. 2011;194(4): 839-54. PMID: 22194455 | PMCID: PMC3272961
  • Rao J, Damron F, Basler M, Digiandomenico A, Sherman N, Fox J, Mekalanos J, Goldberg J. Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient. Frontiers in microbiology. 2011;2 162. PMID: 21863142 | PMCID: PMC3149151
  • Rao J, DiGiandomenico A, Artamonov M, Leitinger N, Amin A, Goldberg J. Host derived inflammatory phospholipids regulate rahU (PA0122) gene, protein, and biofilm formation in Pseudomonas aeruginosa. Cellular immunology. 2011;270(2): 95-102. PMID: 21679933 | PMCID: PMC3415270
  • Rao J, Elliott M, Leitinger N, Jensen R, Goldberg J, Amin A. RahU: an inducible and functionally pleiotropic protein in Pseudomonas aeruginosa modulates innate immunity and inflammation in host cells. Cellular immunology. 2011;270(2): 103-13. PMID: 21704311 | PMCID: PMC3432393
  • Why is Pseudomonas aeruginosa a pathogen? F1000 biology reports. 2010;2. PMID: 20948804 | PMCID: PMC2948353
  • Ding S, Fischer W, Kaparakis-Liaskos M, Liechti G, Merrell D, Grant P, Ferrero R, Crowe S, Haas R, Hatakeyama M, Goldberg J. Helicobacter pylori-induced histone modification, associated gene expression in gastric epithelial cells, and its implication in pathogenesis. PloS one. 2010;5(4): e9875. PMID: 20368982 | PMCID: PMC2848570
  • Ding S, Goldberg J, Hatakeyama M. Helicobacter pylori infection, oncogenic pathways and epigenetic mechanisms in gastric carcinogenesis. Future oncology (London, England). 2010;6(5): 851-62. PMID: 20465395 | PMCID: PMC2882595
  • Oberhardt M, Goldberg J, Hogardt M, Papin J. Metabolic network analysis of Pseudomonas aeruginosa during chronic cystic fibrosis lung infection. Journal of bacteriology. 2010;192(20): 5534-48. PMID: 20709898 | PMCID: PMC2950489
  • Bondi S, Goldberg J. Strategies toward vaccines against Burkholderia mallei and Burkholderia pseudomallei. Expert review of vaccines. 2008;7(9): 1357-65. PMID: 18980539 | PMCID: PMC2680498
  • Ding S, Olekhnovich I, Cover T, Peek R, Smith M, Goldberg J. Helicobacter pylori and mitogen-activated protein kinases mediate activator protein-1 (AP-1) subcomponent protein expression and DNA-binding activity in gastric epithelial cells. FEMS immunology and medical microbiology. 2008;53(3): 385-94. PMID: 18625013 | PMCID: PMC2553690
  • Ding S, Smith M, Goldberg J. Helicobacter pylori and mitogen-activated protein kinases regulate the cell cycle, proliferation and apoptosis in gastric epithelial cells. Journal of gastroenterology and hepatology. 2008;23(7): e67-78. PMID: 18702686
  • Goldberg J, Hancock R, Parales R, Loper J, Cornelis P. Pseudomonas 2007. Journal of bacteriology. 2008;190(8): 2649-62. PMID: 18165299 | PMCID: PMC2293268
  • Kintz E, Goldberg J. Regulation of lipopolysaccharide O antigen expression in Pseudomonas aeruginosa. Future microbiology. 2008;3(2): 191-203. PMID: 18366339
  • Rao J, DiGiandomenico A, Unger J, Bao Y, Polanowska-Grabowska R, Goldberg J. A novel oxidized low-density lipoprotein-binding protein from Pseudomonas aeruginosa. Microbiology (Reading, England). 2008;154 654-65. PMID: 18227268