Edward Perez-Reyes

Education

  • BA, University of North Carolina
  • PhD, University of Colorado, Denver
  • Postdoc, Stanford University

Primary Appointment

  • Professor, Pharmacology

Contact

Research Interest(s)

Exploring epilepsy circuits then preventing seizures using AAV-based gene therapies.

Research Description

Current work in the lab is focused on developing gene therapies for pharmacoresistant epilepsies. In these projects we are leveraging our experience in molecular cloning to develop novel adeno-associated viral (AAV) targeting vectors capable of delivering either short-hairpinned RNAs (shRNAs) or genes of interest. Figure below shows drug-inducible delivery of two genes from a single adeno-associated virus in the brain (GFP and mCherry). In a project funded by a CURE Challenge Award, we validated the ability of a modified K+ leak channel to reduce status epilepticus (Epilepsia: PMID: 24299204). In a second project recently funded by NINDS, we are testing the ability of an shRNA directed against the sodium channel subunits to reduce seizures. Both projects are made possible by collaborations with local epilepsy experts, including Jaideep Kapur, John Williamson, Edward Bertram, Mark Beenhakker, and Manoj Patel.

Selected Publications

  • Dey D, Eckle V, Vitko I, Sullivan K, Lasiecka Z, Winckler B, Stornetta R, Williamson J, Kapur J, Perez-Reyes E. A potassium leak channel silences hyperactive neurons and ameliorates status epilepticus. Epilepsia. 2013;55(2): 203-13. PMID: 24299204 | PMCID: PMC4161023
  • Eckle V, Shcheglovitov A, Vitko I, Dey D, Yap C, Winckler B, Perez-Reyes E. Mechanisms by which a CACNA1H mutation in epilepsy patients increases seizure susceptibility. The Journal of physiology. 2013;592 795-809. PMID: 24277868 | PMCID: PMC3934715
  • Shcheglovitov A, Vitko I, Lazarenko R, Orestes P, Todorovic S, Perez-Reyes E. Molecular and biophysical basis of glutamate and trace metal modulation of voltage-gated Ca(v)2.3 calcium channels. The Journal of general physiology. 2012;139(3): 219-34. PMID: 22371363 | PMCID: PMC3289959
  • Hargus N, Jennings C, Perez-Reyes E, Bertram E, Patel M. Enhanced actions of adenosine in medial entorhinal cortex layer II stellate neurons in temporal lobe epilepsy are mediated via A(1)-receptor activation. Epilepsia. 2011;53(1): 168-76. PMID: 22126400 | PMCID: PMC3253213
  • Characterization of the gating brake in the I-II loop of CaV3 T-type calcium channels. Channels (Austin, Tex.). 2010;4(6): 453-8. PMID: 21099341 | PMCID: PMC3052245
  • Arias-Olguín I, Vitko I, Fortuna M, Baumgart J, Sokolova S, Shumilin I, Van Deusen A, Soriano-García M, Gomora J, Perez-Reyes E. Characterization of the gating brake in the I-II loop of Ca(v)3.2 T-type Ca(2+) channels. The Journal of biological chemistry. 2008;283(13): 8136-44. PMID: 18218623 | PMCID: PMC2276388
  • Baumgart J, Vitko I, Bidaud I, Kondratskyi A, Lory P, Perez-Reyes E. I-II loop structural determinants in the gating and surface expression of low voltage-activated calcium channels. PloS one. 2008;3(8): e2976. PMID: 18714336 | PMCID: PMC2495038
  • Shcheglovitov A, Vitko I, Bidaud I, Baumgart J, Navarro-Gonzalez M, Grayson T, Lory P, Hill C, Perez-Reyes E. Alternative splicing within the I-II loop controls surface expression of T-type Ca(v)3.1 calcium channels. FEBS letters. 2008;582(27): 3765-70. PMID: 18930057 | PMCID: PMC2614674
  • Vitko I, Shcheglovitov A, Baumgart J, Arias-Olguín I, Murbartián J, Arias J, Perez-Reyes E. Orientation of the calcium channel beta relative to the alpha(1)2.2 subunit is critical for its regulation of channel activity. PloS one. 2008;3(10): e3560. PMID: 18958281 | PMCID: PMC2570331
  • Vitko I, Chen Y, Arias J, Shen Y, Wu X, Perez-Reyes E. Functional characterization and neuronal modeling of the effects of childhood absence epilepsy variants of CACNA1H, a T-type calcium channel. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2005;25(19): 4844-55. PMID: 15888660