David L. Brautigan

Education

  • BA, Kalamazoo College, Kalamazoo, MI
  • MS, Northwestern University, Evanston, IL
  • PhD, Northwestern University, Evanston, IL
  • Postdoc, University of Washington School of Medicine, Seattle, WA

Primary Appointment

  • Professor, Microbiology, Immunology, and Cancer Biology

Contact

Research Interest(s)

Protein Phosphorylation in Cell Signaling

Research Description

Protein Phosphorylation in Cell Signaling

The dominant mechanism for cell signaling is protein phosphorylation. Thousands of cellular proteins undergo phosphorylation to control their activity, but most of the time they are kept in their dephosphorylated state by the action of protein phosphatases. Phosphatases specific for P-Ser and P-Thr (PP1, PP2A, PP4, PP6) are ancient enzymes, conserved between yeast and humans, and genetics shows they are essential for mitosis and for stages of cell cycle growth and cell proliferation.

Toxins from cyanobacteria, dinoflagelates and insects target the active sites of these phosphatases. Cells must strictly regulate the activity of these abundant phosphatases to respond to changes in their environment. This is done through sending signals to phosphatase regulatory and catalytic subunits, plus specific inhibitor proteins. Current research in our group investigates the molecular basis for recognition among these proteins, and tests how phosphatases operate in living cells in response to changing conditions.

We are determining the structure and biochemical properties of purified recombinant proteins. We use genomics to find novel related proteins and genetic screens to find binding partners. Fluorescent fusion proteins and phosphorylation-specific antibodies are employed with microscopic digital imaging to study where, when and how proteins become activated and localized within cells. The results are expected to give a better understanding of cell division, cytoskeletal reorganization, metabolic regulation and how defects in these processes lead to human diseases. [see website of Center for Cell Signaling for more detailed description of research projects: https://research.med.virginia.edu/cell-signaling/]

Selected Publications

  • McClinch K, A Avelar R, Callejas D, Izadmehr S, Wiredja D, Perl A, Sangodkar J, Kastrinsky D, Schlatzer D, Cooper M, Kiselar J, Stachnik A, Yao S, Hoon D, McQuaid D, Zaware N, Gong Y, Brautigan D, Plymate S, Sprenger C, Oh W, Levine A, Kirschenbaum A, Sfakianos J, Sears R, DiFeo A, Ioannou Y, Ohlmeyer M, Narla G, Galsky M. Small molecule activators of protein phosphatase 2A for the treatment of castration-resistant prostate cancer. Cancer research. 2018. PMID: 29358171
  • Tong Y, Park S, Wu D, Harris T, Moskaluk C, Brautigan D, Fu Z. Modulation of GSK3β autoinhibition by Thr-7 and Thr-8. FEBS letters. 2018. PMID: 29377106
  • Biswas K, Sarkar S, Du K, Brautigan D, Abbas T, Larner J. The E3 Ligase CHIP Mediates p21 Degradation to Maintain Radioresistance. Molecular cancer research : MCR. 2017;15(6): 651-659. PMID: 28232384 | PMCID: PMC5687089
  • Brautigan D, Li R, Kubicka E, Turner S, Garcia J, Weintraut M, Wong E. Lysolecithin as feed additive enhances collagen expression and villus length in the jejunum of broiler chickens. Poultry science. 2017;96(8): 2889-2898. PMID: 28444343
  • Li R, Turner S, Brautigan D. Xanthophylls lutein and zeaxanthin modify gene expression and induce synthesis of hyaluronan in keratinocyte model of human skin. Biochemistry and biophysics reports. 2017;4 52-58. PMID: 29124187 | PMCID: PMC5668877
  • Sangodkar J, Perl A, Tohme R, Kiselar J, Kastrinsky D, Zaware N, Izadmehr S, Mazhar S, Wiredja D, O'Connor C, Hoon D, Dhawan N, Schlatzer D, Yao S, Leonard D, Borczuk A, Gokulrangan G, Wang L, Svenson E, Farrington C, Yuan E, Avelar R, Stachnik A, Smith B, Gidwani V, Giannini H, McQuaid D, McClinch K, Wang Z, Levine A, Sears R, Chen E, Duan Q, Datt M, Haider S, Ma'ayan A, DiFeo A, Sharma N, Galsky M, Brautigan D, Ioannou Y, Xu W, Chance M, Ohlmeyer M, Narla G. Activation of tumor suppressor protein PP2A inhibits KRAS-driven tumor growth. The Journal of clinical investigation. 2017;127(6): 2081-2090. PMID: 28504649 | PMCID: PMC5451217
  • Sarkar S, Brautigan D, Larner J. Aurora Kinase A Promotes AR Degradation via the E3 Ligase CHIP. Molecular cancer research : MCR. 2017;15(8): 1063-1072. PMID: 28536143 | PMCID: PMC5697428
  • Shah M, Smolko C, Kinicki S, Chapman Z, Brautigan D, Janes K. Profiling Subcellular Protein Phosphatase Responses to Coxsackievirus B3 Infection of Cardiomyocytes. Molecular & cellular proteomics : MCP. 2017;16(4): S244-S262. PMID: 28174228 | PMCID: PMC5393398
  • Ziembik M, Bender T, Larner J, Brautigan D. Functions of protein phosphatase-6 in NF-κB signaling and in lymphocytes. Biochemical Society transactions. 2017;45(3): 693-701. PMID: 28620030 | PMCID: PMC5473023
  • Farinha C, Swiatecka-Urban A, Brautigan D, Jordan P. Regulatory Crosstalk by Protein Kinases on CFTR Trafficking and Activity. Frontiers in chemistry. 2016;4 1. PMID: 26835446 | PMCID: PMC4718993
  • Wilkins L, Brautigan D, Wu H, Yarmohammadi H, Kubicka E, Serbulea V, Leitinger N, Liu W, Haaga J. Cinnamic Acid Derivatives Enhance the Efficacy of Transarterial Embolization in a Rat Model of Hepatocellular Carcinoma. Cardiovascular and interventional radiology. 2016. PMID: 27872984
  • Griner E, Dancik G, Costello J, Owens C, Guin S, Edwards M, Brautigan D, Theodorescu D. RhoC Is an Unexpected Target of RhoGDI2 in Prevention of Lung Colonization of Bladder Cancer. Molecular cancer research : MCR. 2014;13(3): 483-92. PMID: 25516960 | PMCID: PMC4369172
  • Hall E, Liu Y, Xiao A, Shock L, Brautigan D, Mayo M, Adusumilli P, Jones D. Inhibition of breast cancer metastasis suppressor 1 promotes a mesenchymal phenotype in lung epithelial cells that express oncogenic K-RasV12 and loss of p53. PloS one. 2014;9(4): e95869. PMID: 24763730 | PMCID: PMC3999110
  • Luz S, Cihil K, Brautigan D, Amaral M, Farinha C, Swiatecka-Urban A. LMTK2-mediated phosphorylation regulates CFTR endocytosis in human airway epithelial cells. The Journal of biological chemistry. 2014;289(21): 15080-93. PMID: 24727471 | PMCID: PMC4031558
  • Yan C, Liu D, Li L, Wempe M, Guin S, Khanna M, Meier J, Hoffman B, Owens C, Wysoczynski C, Nitz M, Knabe E, Brautigan D, Paschal B, Schwartz M, Jones D, Ross D, Meroueh S, Theodorescu D. Discovery and characterization of small molecules that target the GTPase Ral. Nature. 2014. PMID: 25219851 | PMCID: PMC4351747
  • Dziegielewska B, Brautigan D, Larner J, Dziegielewski J. T-type Ca2+ Channel Inhibition Induces p53 Dependent Cell Growth Arrest and Apoptosis through Activation of p38-MAPK in Colon Cancer Cells. Molecular cancer research : MCR. 2013. PMID: 24362252
  • Korrodi-Gregório L, Ferreira M, Vintém A, Wu W, Muller T, Marcus K, Vijayaraghavan S, Brautigan D, da Cruz E Silva O, Fardilha M, da Cruz E Silva E. Identification and characterization of two distinct PPP1R2 isoforms in human spermatozoa. BMC cell biology. 2013;14 15. PMID: 23506001 | PMCID: PMC3606321
  • Martín-Montalvo A, González-Mariscal I, Pomares-Viciana T, Padilla-López S, Ballesteros M, Vazquez-Fonseca L, Gandolfo P, Brautigan D, Navas P, Santos-Ocaña C. The phosphatase Ptc7 induces coenzyme Q biosynthesis by activating the hydroxylase Coq7 in yeast. The Journal of biological chemistry. 2013;288(39): 28126-37. PMID: 23940037 | PMCID: PMC3784724
  • Ohama T, Wang L, Griner E, Brautigan D. Protein Ser/Thr phosphatase-6 is required for maintenance of E-cadherin at adherens junctions. BMC cell biology. 2013;14 42. PMID: 24063632 | PMCID: PMC3856536
  • Slingluff C, Petroni G, Molhoek K, Brautigan D, Chianese-Bullock K, Shada A, Smolkin M, Olson W, Gaucher A, Chase C, Grosh W, Weiss G, Wagenseller A, Olszanski A, Martin L, Shea S, Erdag G, Ram P, Gershenwald J, Weber M. Clinical activity and safety of combination therapy with temsirolimus and bevacizumab for advanced melanoma: a phase II trial (CTEP 7190/Mel47). Clinical cancer research : an official journal of the American Association for Cancer Research. 2013;19(13): 3611-20. PMID: 23620404 | PMCID: PMC3700572
  • Valerie N, Dziegielewska B, Hosing A, Augustin E, Gray L, Brautigan D, Larner J, Dziegielewski J. Inhibition of T-type calcium channels disrupts Akt signaling and promotes apoptosis in glioblastoma cells. Biochemical pharmacology. 2013;85(7): 888-97. PMID: 23287412
  • Wang L, Brautigan D. α-SNAP inhibits AMPK signaling to reduce mitochondrial biogenesis and dephosphorylates Thr172 in AMPKα in vitro. Nature communications. 2013;4 1559. PMID: 23463002 | PMCID: PMC3595137
  • Protein Ser/ Thr phosphatases - the ugly ducklings of cell signalling. The FEBS journal. 2012. PMID: 22519956
  • Eto M, Brautigan D. Endogenous inhibitor proteins that connect Ser/Thr kinases and phosphatases in cell signaling. IUBMB life. 2012;64(9): 732-9. PMID: 22815089 | PMCID: PMC3422418
  • Griner E, Churchill M, Brautigan D, Theodorescu D. PKCα phosphorylation of RhoGDI2 at Ser31 disrupts interactions with Rac1 and decreases GDI activity. Oncogene. 2012. PMID: 22469974 | PMCID: PMC3578973
  • Holy M, Brautigan D. Calyculin A from Discodermia calyx is a dual action toxin that blocks calcium influx and inhibits protein Ser/Thr phosphatases. Toxins. 2012;4(10): 940-54. PMID: 23162706 | PMCID: PMC3496997
  • Hosing A, Valerie N, Dziegielewski J, Brautigan D, Larner J. PP6 regulatory subunit R1 is bidentate anchor for targeting protein phosphatase-6 to DNA-dependent protein kinase. The Journal of biological chemistry. 2012;287(12): 9230-9. PMID: 22298787 | PMCID: PMC3308816
  • Pitt J, Thorner M, Brautigan D, Larner J, Klein W. Protection against the synaptic targeting and toxicity of Alzheimer's-associated Aβ oligomers by insulin mimetic chiro-inositols. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2012;27(1): 199-207. PMID: 23073831 | PMCID: PMC3528307
  • Sarkar S, Brautigan D, Parsons S, Larner J. Androgen receptor degradation by the E3 ligase CHIP modulates mitotic arrest in prostate cancer cells. Oncogene. 2012;33(1): 26-33. PMID: 23246967 | PMCID: PMC3899837
  • Edelson J, Brautigan D. The Discodermia calyx Toxin Calyculin A Enhances Cyclin D1 Phosphorylation and Degradation, and Arrests Cell Cycle Progression in Human Breast Cancer Cells. Toxins. 2011;3(1): 105-19. PMID: 22069692 | PMCID: PMC3210456
  • Everett A, Yang J, Rahman M, Dulloor P, Brautigan D. Mitotic phosphorylation activates hepatoma-derived growth factor as a mitogen. BMC cell biology. 2011;12 15. PMID: 21489262 | PMCID: PMC3094319
  • Hall E, Gurel V, Dahlberg A, McMichael J, Brautigan D. Inhibition of human breast cancer Matrigel invasion by Streptolysin O activation of the EGF receptor ErbB1. Cellular signalling. 2011;23(12): 1972-7. PMID: 21787862 | PMCID: NIHMS312507
  • Martin-Montalvo A, Gonzalez-Mariscal I, Padilla S, Ballesteros M, Brautigan D, Navas P, Santos-Ocaña C. Respiratory-induced coenzyme Q biosynthesis is regulated by a phosphorylation cycle of Cat5p/Coq7p. The Biochemical journal. 2011. PMID: 21812761
  • Molhoek K, Erdag G, Rasamny J, Murphy C, Deacon D, Patterson J, Slingluff C, Brautigan D. VEGFR-2 expression in human melanoma: revised assessment. International journal of cancer. Journal international du cancer. 2011;129(12): 2807-15. PMID: 21544800 | PMCID: PMC3205910
  • Molhoek K, Shada A, Smolkin M, Chowbina S, Papin J, Brautigan D, Slingluff C. Comprehensive analysis of receptor tyrosine kinase activation in human melanomas reveals autocrine signaling through IGF-1R. Melanoma research. 2011;21(4): 274-84. PMID: 21654344 | PMCID: PMC3131461
  • Sami F, Smet-Nocca C, Khan M, Landrieu I, Lippens G, Brautigan D. Molecular basis for an ancient partnership between prolyl isomerase Pin1 and phosphatase inhibitor-2. Biochemistry. 2011;50(30): 6567-78. PMID: 21714498 | PMCID: PMC3965691
  • Hall E, Balsbaugh J, Rose K, Shabanowitz J, Hunt D, Brautigan D. Comprehensive analysis of phosphorylation sites in Tensin1 reveals regulation by p38MAPK. Molecular & cellular proteomics : MCP. 2010;9(12): 2853-63. PMID: 20798394 | PMCID: PMC3101865
  • Hans S, Camara F, Altiti A, Martín-Montalvo A, Brautigan D, Heimark D, Larner J, Grindrod S, Brown M, Mootoo D. Synthesis of C-glycoside analogues of beta-galactosamine-(1-->4)-3-O-methyl-D-chiro-inositol and assay as activator of protein phosphatases PDHP and PP2Calpha. Bioorganic & medicinal chemistry. 2010;18(3): 1103-10. PMID: 20079654 | PMCID: PMC3408221
  • Larner J, Brautigan D, Thorner M. D-chiro-inositol glycans in insulin signaling and insulin resistance. Molecular medicine (Cambridge, Mass.). 2010;16(11): 543-52. PMID: 20811656 | PMCID: PMC2972396
  • Ohama T, Brautigan D. Endotoxin conditioning induces VCP/p97-mediated and inducible nitric-oxide synthase-dependent Tyr284 nitration in protein phosphatase 2A. The Journal of biological chemistry. 2010;285(12): 8711-8. PMID: 20100830 | PMCID: PMC2838294
  • Wang H, Owens C, Chandra N, Conaway M, Brautigan D, Theodorescu D. Phosphorylation of RalB is important for bladder cancer cell growth and metastasis. Cancer research. 2010;70(21): 8760-9. PMID: 20940393 | PMCID: PMC3025490
  • Broglie P, Matsumoto K, Akira S, Brautigan D, Ninomiya-Tsuji J. Transforming growth factor beta-activated kinase 1 (TAK1) kinase adaptor, TAK1-binding protein 2, plays dual roles in TAK1 signaling by recruiting both an activator and an inhibitor of TAK1 kinase in tumor necrosis factor signaling pathway. The Journal of biological chemistry. 2009;285(4): 2333-9. PMID: 19955178 | PMCID: PMC2807291
  • Guergnon J, Derewenda U, Edelson J, Brautigan D. Mapping of protein phosphatase-6 association with its SAPS domain regulatory subunit using a model of helical repeats. BMC biochemistry. 2009;10 24. PMID: 19835610 | PMCID: PMC2765987
  • Hall E, Daugherty A, Choi C, Horwitz A, Brautigan D. Tensin1 requires protein phosphatase-1alpha in addition to RhoGAP DLC-1 to control cell polarization, migration, and invasion. The Journal of biological chemistry. 2009;284(50): 34713-22. PMID: 19826001 | PMCID: PMC2787334
  • Mi J, Bolesta E, Brautigan D, Larner J. PP2A regulates ionizing radiation-induced apoptosis through Ser46 phosphorylation of p53. Molecular cancer therapeutics. 2009;8(1): 135-40. PMID: 19139122
  • Mi J, Dziegielewski J, Bolesta E, Brautigan D, Larner J. Activation of DNA-PK by ionizing radiation is mediated by protein phosphatase 6. PloS one. 2009;4(2): e4395. PMID: 19198648 | PMCID: PMC2634843
  • Morales-Johansson H, Puria R, Brautigan D, Cardenas M. Human protein phosphatase PP6 regulatory subunits provide Sit4-dependent and rapamycin-sensitive sap function in Saccharomyces cerevisiae. PloS one. 2009;4(7): e6331. PMID: 19621075 | PMCID: PMC2708350
  • Fuller B, Lampson M, Foley E, Rosasco-Nitcher S, Le K, Tobelmann P, Brautigan D, Stukenberg P, Kapoor T. Midzone activation of aurora B in anaphase produces an intracellular phosphorylation gradient. Nature. 2008;453(7198): 1132-6. PMID: 18463638 | PMCID: PMC2724008
  • Molhoek K, Griesemann H, Shu J, Gershenwald J, Brautigan D, Slingluff C. Human melanoma cytolysis by combined inhibition of mammalian target of rapamycin and vascular endothelial growth factor/vascular endothelial growth factor receptor-2. Cancer research. 2008;68(11): 4392-7. PMID: 18519701 | PMCID: PMC2727753
  • Molhoek K, McSkimming C, Olson W, Brautigan D, Slingluff C. Apoptosis of CD4(+)CD25(high) T cells in response to Sirolimus requires activation of T cell receptor and is modulated by IL-2. Cancer immunology, immunotherapy : CII. 2008;58(6): 867-76. PMID: 18841360 | PMCID: PMC2688807
  • Ohama T, Okada M, Murata T, Brautigan D, Hori M, Ozaki H. Sphingosine-1-phosphate enhances IL-1{beta}-induced COX-2 expression in mouse intestinal subepithelial myofibroblasts. American journal of physiology. Gastrointestinal and liver physiology. 2008;295(4): G766-75. PMID: 18703638
  • Prickett T, Ninomiya-Tsuji J, Broglie P, Muratore-Schroeder T, Shabanowitz J, Hunt D, Brautigan D. TAB4 stimulates TAK1-TAB1 phosphorylation and binds polyubiquitin to direct signaling to NF-kappaB. The Journal of biological chemistry. 2008;283(28): 19245-54. PMID: 18456659 | PMCID: PMC2443674
  • Stefansson B, Ohama T, Daugherty A, Brautigan D. Protein phosphatase 6 regulatory subunits composed of ankyrin repeat domains. Biochemistry. 2008;47(5): 1442-51. PMID: 18186651
  • Wang W, Brautigan D. Phosphatase inhibitor 2 promotes acetylation of tubulin in the primary cilium of human retinal epithelial cells. BMC cell biology. 2008;9 62. PMID: 19036150 | PMCID: PMC2630314
  • Wang W, Cronmiller C, Brautigan D. Maternal phosphatase inhibitor-2 is required for proper chromosome segregation and mitotic synchrony during Drosophila embryogenesis. Genetics. 2008;179(4): 1823-33. PMID: 18689877 | PMCID: PMC2516061
  • Wang W, Stukenberg P, Brautigan D. Phosphatase inhibitor-2 balances protein phosphatase 1 and aurora B kinase for chromosome segregation and cytokinesis in human retinal epithelial cells. Molecular biology of the cell. 2008;19(11): 4852-62. PMID: 18716057 | PMCID: PMC2575180
  • Yoo S, Jimenez R, Sanders J, Boylan J, Brautigan D, Gruppuso P. The alpha4-containing form of protein phosphatase 2A in liver and hepatic cells. Journal of cellular biochemistry. 2008;105(1): 290-300. PMID: 18543252 | PMCID: PMC2597445
  • Eto M, Brautigan D. Assay for three-way interaction of protein phosphatase-1 (Glc7) with regulatory subunits plus phosphatase inhibitor-2. Methods in molecular biology (Clifton, N.J.). 2007;365 197-208. PMID: 17200563
  • Eto M, Kirkbride J, Elliott E, Lo S, Brautigan D. Association of the tensin N-terminal protein-tyrosine phosphatase domain with the alpha isoform of protein phosphatase-1 in focal adhesions. The Journal of biological chemistry. 2007;282(24): 17806-15. PMID: 17435217
  • Eto M, Kitazawa T, Matsuzawa F, Aikawa S, Kirkbride J, Isozumi N, Nishimura Y, Brautigan D, Ohki S. Phosphorylation-induced conformational switching of CPI-17 produces a potent myosin phosphatase inhibitor. Structure (London, England : 1993). 2007;15(12): 1591-602. PMID: 18073109 | PMCID: PMC2217667
  • Li M, Satinover D, Brautigan D. Phosphorylation and functions of inhibitor-2 family of proteins. Biochemistry. 2007;46(9): 2380-9. PMID: 17286390
  • Li M, Stukenberg P, Brautigan D. Binding of phosphatase inhibitor-2 to prolyl isomerase Pin1 modifies specificity for mitotic phosphoproteins. Biochemistry. 2007;47(1): 292-300. PMID: 18062707
  • Mi J, Guo C, Brautigan D, Larner J. Protein phosphatase-1alpha regulates centrosome splitting through Nek2. Cancer research. 2007;67(3): 1082-9. PMID: 17283141
  • Prickett T, Brautigan D. Cytokine activation of p38 mitogen-activated protein kinase and apoptosis is opposed by alpha-4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3. Molecular and cellular biology. 2007;27(12): 4217-27. PMID: 17438131 | PMCID: PMC1900036
  • Smith S, Oxford G, Baras A, Owens C, Havaleshko D, Brautigan D, Safo M, Theodorescu D. Expression of ral GTPases, their effectors, and activators in human bladder cancer. Clinical cancer research : an official journal of the American Association for Cancer Research. 2007;13(13): 3803-13. PMID: 17606711
  • Stefansson B, Brautigan D. Protein phosphatase PP6 N terminal domain restricts G1 to S phase progression in human cancer cells. Cell cycle (Georgetown, Tex.). 2007;6(11): 1386-92. PMID: 17568194
  • Yang C, Xin H, Kelley J, Spencer A, Brautigan D, Paschal B. Ligand binding to the androgen receptor induces conformational changes that regulate phosphatase interactions. Molecular and cellular biology. 2007;27(9): 3390-404. PMID: 17325038 | PMCID: PMC1899975
  • Yang J, Roe S, Prickett T, Brautigan D, Barford D. The structure of Tap42/alpha4 reveals a tetratricopeptide repeat-like fold and provides insights into PP2A regulation. Biochemistry. 2007;46(30): 8807-15. PMID: 17616149
  • Yoo S, Boylan J, Brautigan D, Gruppuso P. Subunit composition and developmental regulation of hepatic protein phosphatase 2A (PP2A). Archives of biochemistry and biophysics. 2007;461(2): 186-93. PMID: 17391644 | PMCID: PMC1868455
  • Brautigan D, Kruszewski A, Wang H. Chromium and vanadate combination increases insulin-induced glucose uptake by 3T3-L1 adipocytes. Biochemical and biophysical research communications. 2006;347(3): 769-73. PMID: 16842748
  • Gianchandani E, Brautigan D, Papin J. Systems analyses characterize integrated functions of biochemical networks. Trends in biochemical sciences. 2006;31(5): 284-91. PMID: 16616498
  • Guo C, Mi J, Brautigan D, Larner J. ATM regulates ionizing radiation-induced disruption of HDAC1:PP1:Rb complexes. Cellular signalling. 2006;19(3): 504-10. PMID: 17008050
  • Kajino T, Ren H, Iemura S, Natsume T, Stefansson B, Brautigan D, Matsumoto K, Ninomiya-Tsuji J. Protein phosphatase 6 down-regulates TAK1 kinase activation in the IL-1 signaling pathway. The Journal of biological chemistry. 2006;281(52): 39891-6. PMID: 17079228 | PMCID: PMC1797071
  • Prickett T, Brautigan D. The alpha4 regulatory subunit exerts opposing allosteric effects on protein phosphatases PP6 and PP2A. The Journal of biological chemistry. 2006;281(41): 30503-11. PMID: 16895907
  • Satinover D, Brautigan D, Stukenberg P. Aurora-A kinase and inhibitor-2 regulate the cyclin threshold for mitotic entry in Xenopus early embryonic cell cycles. Cell cycle (Georgetown, Tex.). 2006;5(19): 2268-74. PMID: 16969136
  • Stefansson B, Brautigan D. Protein phosphatase 6 subunit with conserved Sit4-associated protein domain targets IkappaBepsilon. The Journal of biological chemistry. 2006;281(32): 22624-34. PMID: 16769727
  • Swain J, Ding J, Brautigan D, Villa-Moruzzi E, Smith G. Proper chromatin condensation and maintenance of histone H3 phosphorylation during mouse oocyte meiosis requires protein phosphatase activity. Biology of reproduction. 2006;76(4): 628-38. PMID: 17182892
  • Wang H, Brautigan D. Peptide microarray analysis of substrate specificity of the transmembrane Ser/Thr kinase KPI-2 reveals reactivity with cystic fibrosis transmembrane conductance regulator and phosphorylase. Molecular & cellular proteomics : MCP. 2006;5(11): 2124-30. PMID: 16887929
  • Wu Y, Wang H, Brautigan D, Liu Z. Activation of glycogen synthase in myocardium induced by intermittent hypoxia is much lower in fasted than in fed rats. American journal of physiology. Endocrinology and metabolism. 2006;292(2): E469-75. PMID: 17003235
  • Brautigan D, Brown M, Grindrod S, Chinigo G, Kruszewski A, Lukasik S, Bushweller J, Horal M, Keller S, Tamura S, Heimark D, Price J, Larner A, Larner J. Allosteric activation of protein phosphatase 2C by D-chiro-inositol-galactosamine, a putative mediator mimetic of insulin action. Biochemistry. 2005;44(33): 11067-73. PMID: 16101290
  • Eto M, Kirkbride J, Brautigan D. Assembly of MYPT1 with protein phosphatase-1 in fibroblasts redirects localization and reorganizes the actin cytoskeleton. Cell motility and the cytoskeleton. 2005;62(2): 100-9. PMID: 16106448
  • Li M, Stefansson B, Wang W, Schaefer E, Brautigan D. Phosphorylation of the Pro-X-Thr-Pro site in phosphatase inhibitor-2 by cyclin-dependent protein kinase during M-phase of the cell cycle. Cellular signalling. 2005;18(8): 1318-26. PMID: 16377132
  • Molhoek K, Brautigan D, Slingluff C. Synergistic inhibition of human melanoma proliferation by combination treatment with B-Raf inhibitor BAY43-9006 and mTOR inhibitor Rapamycin. Journal of translational medicine. 2005;3 39. PMID: 16255777 | PMCID: PMC1289294
  • Wang H, Kruszewski A, Brautigan D. Cellular chromium enhances activation of insulin receptor kinase. Biochemistry. 2005;44(22): 8167-75. PMID: 15924436
  • Yang C, Vitto M, Busby S, Garcia B, Kesler C, Gioeli D, Shabanowitz J, Hunt D, Rundell K, Brautigan D, Paschal B. Simian virus 40 small t antigen mediates conformation-dependent transfer of protein phosphatase 2A onto the androgen receptor. Molecular and cellular biology. 2005;25(4): 1298-308. PMID: 15684382 | PMCID: PMC548022
  • Eto M, Kitazawa T, Brautigan D. Phosphoprotein inhibitor CPI-17 specificity depends on allosteric regulation of protein phosphatase-1 by regulatory subunits. Proceedings of the National Academy of Sciences of the United States of America. 2004;101(24): 8888-93. PMID: 15184667 | PMCID: PMC428442
  • Prickett T, Brautigan D. Overlapping binding sites in protein phosphatase 2A for association with regulatory A and alpha-4 (mTap42) subunits. The Journal of biological chemistry. 2004;279(37): 38912-20. PMID: 15252037
  • Satinover D, Leach C, Stukenberg P, Brautigan D. Activation of Aurora-A kinase by protein phosphatase inhibitor-2, a bifunctional signaling protein. Proceedings of the National Academy of Sciences of the United States of America. 2004;101(23): 8625-30. PMID: 15173575 | PMCID: PMC423245
  • Tountas N, Brautigan D. Migration and retraction of endothelial and epithelial cells require PHI-1, a specific protein-phosphatase-1 inhibitor protein. Journal of cell science. 2004;117 5905-12. PMID: 15522888
  • Tountas N, Mandell J, Everett A, Brautigan D. Juxtamembrane localization of the protein phosphatase-1 inhibitor protein PHI-1 in smooth muscle cells. Histochemistry and cell biology. 2004;121(4): 343-50. PMID: 15083373
  • Erdodi F, Kiss E, Walsh M, Stefansson B, Deng J, Eto M, Brautigan D, Hartshorne D. Phosphorylation of protein phosphatase type-1 inhibitory proteins by integrin-linked kinase and cyclic nucleotide-dependent protein kinases. Biochemical and biophysical research communications. 2003;306(2): 382-7. PMID: 12804574
  • Eto M, Leach C, Tountas N, Brautigan D. Phosphoprotein inhibitors of protein phosphatase-1. Methods in enzymology. 2003;366 243-60. PMID: 14674253
  • Leach C, Shenolikar S, Brautigan D. Phosphorylation of phosphatase inhibitor-2 at centrosomes during mitosis. The Journal of biological chemistry. 2003;278(28): 26015-20. PMID: 12697755
  • Murthy K, Zhou H, Grider J, Brautigan D, Eto M, Makhlouf G. Differential signalling by muscarinic receptors in smooth muscle: m2-mediated inactivation of myosin light chain kinase via Gi3, Cdc42/Rac1 and p21-activated kinase 1 pathway, and m3-mediated MLC20 (20 kDa regulatory light chain of myosin II) phosphorylation via Rho-associated kinase/myosin phosphatase targeting subunit 1 and protein kinase C/CPI-17 pathway. The Biochemical journal. 2003;374 145-55. PMID: 12733988 | PMCID: PMC1223565
  • Ohki S, Eto M, Shimizu M, Takada R, Brautigan D, Kainosho M. Distinctive solution conformation of phosphatase inhibitor CPI-17 substituted with aspartate at the phosphorylation-site threonine residue. Journal of molecular biology. 2003;326(5): 1539-47. PMID: 12595264
  • Deng J, Sutherland C, Brautigan D, Eto M, Walsh M. Phosphorylation of the myosin phosphatase inhibitors, CPI-17 and PHI-1, by integrin-linked kinase. The Biochemical journal. 2002;367 517-24. PMID: 12144526 | PMCID: PMC1222907
  • Eto M, Bock R, Brautigan D, Linden D. Cerebellar long-term synaptic depression requires PKC-mediated activation of CPI-17, a myosin/moesin phosphatase inhibitor. Neuron. 2002;36(6): 1145-58. PMID: 12495628
  • Eto M, Elliott E, Prickett T, Brautigan D. Inhibitor-2 regulates protein phosphatase-1 complexed with NimA-related kinase to induce centrosome separation. The Journal of biological chemistry. 2002;277(46): 44013-20. PMID: 12221103
  • Everett A, Brautigan D. Developmental expression of alpha4 protein phosphatase regulatory subunit in tissues affected by Opitz syndrome. Developmental dynamics : an official publication of the American Association of Anatomists. 2002;224(4): 461-4. PMID: 12203739
  • Everett A, Kamibayashi C, Brautigan D. Transgenic expression of protein phosphatase 2A regulatory subunit B56gamma disrupts distal lung differentiation. American journal of physiology. Lung cellular and molecular physiology. 2002;282(6): L1266-71. PMID: 12003782
  • Guo C, Brautigan D, Larner J. Ionizing radiation activates nuclear protein phosphatase-1 by ATM-dependent dephosphorylation. The Journal of biological chemistry. 2002;277(44): 41756-61. PMID: 12202491
  • Leach C, Eto M, Brautigan D. Domains of type 1 protein phosphatase inhibitor-2 required for nuclear and cytoplasmic localization in response to cell-cell contact. Journal of cell science. 2002;115 3739-45. PMID: 12235284
  • Oliver C, Terry-Lorenzo R, Elliott E, Bloomer W, Li S, Brautigan D, Colbran R, Shenolikar S. Targeting protein phosphatase 1 (PP1) to the actin cytoskeleton: the neurabin I/PP1 complex regulates cell morphology. Molecular and cellular biology. 2002;22(13): 4690-701. PMID: 12052877 | PMCID: PMC133892
  • Terry-Lorenzo R, Elliot E, Weiser D, Prickett T, Brautigan D, Shenolikar S. Neurabins recruit protein phosphatase-1 and inhibitor-2 to the actin cytoskeleton. The Journal of biological chemistry. 2002;277(48): 46535-43. PMID: 12270929
  • Wang H, Brautigan D. A novel transmembrane Ser/Thr kinase complexes with protein phosphatase-1 and inhibitor-2. The Journal of biological chemistry. 2002;277(51): 49605-12. PMID: 12393858
  • Zhou B, Wang Z, Zhao Y, Brautigan D, Zhang Z. The specificity of extracellular signal-regulated kinase 2 dephosphorylation by protein phosphatases. The Journal of biological chemistry. 2002;277(35): 31818-25. PMID: 12082107
  • Broustas C, Grammatikakis N, Eto M, Dent P, Brautigan D, Kasid U. Phosphorylation of the myosin-binding subunit of myosin phosphatase by Raf-1 and inhibition of phosphatase activity. The Journal of biological chemistry. 2001;277(4): 3053-9. PMID: 11719507
  • Eto M, Kitazawa T, Yazawa M, Mukai H, Ono Y, Brautigan D. Histamine-induced vasoconstriction involves phosphorylation of a specific inhibitor protein for myosin phosphatase by protein kinase C alpha and delta isoforms. The Journal of biological chemistry. 2001;276(31): 29072-8. PMID: 11397799
  • Etter E, Eto M, Wardle R, Brautigan D, Murphy R. Activation of myosin light chain phosphatase in intact arterial smooth muscle during nitric oxide-induced relaxation. The Journal of biological chemistry. 2001;276(37): 34681-5. PMID: 11461918
  • Everett A, Stoops T, Nairn A, Brautigan D. Angiotensin II regulates phosphorylation of translation elongation factor-2 in cardiac myocytes. American journal of physiology. Heart and circulatory physiology. 2001;281(1): H161-7. PMID: 11406481
  • Guo C, Brautigan D, Larner J. ATM-dependent dissociation of B55 regulatory subunit from nuclear PP2A in response to ionizing radiation. The Journal of biological chemistry. 2001;277(7): 4839-44. PMID: 11723136
  • Hayashi Y, Senba S, Yazawa M, Brautigan D, Eto M. Defining the structural determinants and a potential mechanism for inhibition of myosin phosphatase by the protein kinase C-potentiated inhibitor protein of 17 kDa. The Journal of biological chemistry. 2001;276(43): 39858-63. PMID: 11517233
  • Liu J, Prickett T, Elliott E, Meroni G, Brautigan D. Phosphorylation and microtubule association of the Opitz syndrome protein mid-1 is regulated by protein phosphatase 2A via binding to the regulatory subunit alpha 4. Proceedings of the National Academy of Sciences of the United States of America. 2001;98(12): 6650-5. PMID: 11371618 | PMCID: PMC34408
  • MacDonald J, Eto M, Borman M, Brautigan D, Haystead T. Dual Ser and Thr phosphorylation of CPI-17, an inhibitor of myosin phosphatase, by MYPT-associated kinase. FEBS letters. 2001;493(2): 91-4. PMID: 11287002
  • Ohki S, Eto M, Kariya E, Hayano T, Hayashi Y, Yazawa M, Brautigan D, Kainosho M. Solution NMR structure of the myosin phosphatase inhibitor protein CPI-17 shows phosphorylation-induced conformational changes responsible for activation. Journal of molecular biology. 2001;314(4): 839-49. PMID: 11734001
  • Shenolikar S, Brautigan D. Meeting report: targeting protein phosphatases-medicines for the new millenium. Science's STKE : signal transduction knowledge environment. 2001;2000(57): pe1. PMID: 11752618
  • Woodsome T, Eto M, Everett A, Brautigan D, Kitazawa T. Expression of CPI-17 and myosin phosphatase correlates with Ca(2+) sensitivity of protein kinase C-induced contraction in rabbit smooth muscle. The Journal of physiology. 2001;535 553-64. PMID: 11533144 | PMCID: PMC2278797
  • Eto M, Wong L, Yazawa M, Brautigan D. Inhibition of myosin/moesin phosphatase by expression of the phosphoinhibitor protein CPI-17 alters microfilament organization and retards cell spreading. Cell motility and the cytoskeleton. 2000;46(3): 222-34. PMID: 10913968
  • Kitazawa T, Eto M, Woodsome T, Brautigan D. Agonists trigger G protein-mediated activation of the CPI-17 inhibitor phosphoprotein of myosin light chain phosphatase to enhance vascular smooth muscle contractility. The Journal of biological chemistry. 2000;275(14): 9897-900. PMID: 10744661
  • Liu J, Brautigan D. Glycogen synthase association with the striated muscle glycogen-targeting subunit of protein phosphatase-1. Synthase activation involves scaffolding regulated by beta-adrenergic signaling. The Journal of biological chemistry. 2000;275(34): 26074-81. PMID: 10856301
  • Liu J, Brautigan D. Insulin-stimulated phosphorylation of the protein phosphatase-1 striated muscle glycogen-targeting subunit and activation of glycogen synthase. The Journal of biological chemistry. 2000;275(21): 15940-7. PMID: 10748124
  • Liu J, Wu J, Oliver C, Shenolikar S, Brautigan D. Mutations of the serine phosphorylated in the protein phosphatase-1-binding motif in the skeletal muscle glycogen-targeting subunit. The Biochemical journal. 2000;346 77-82. PMID: 10657242 | PMCID: PMC1220825
  • Chung H, Brautigan D. Protein phosphatase 2A suppresses MAP kinase signalling and ectopic protein expression. Cellular signalling. 1999;11(8): 575-80. PMID: 10433518
  • Chung H, Nairn A, Murata K, Brautigan D. Mutation of Tyr307 and Leu309 in the protein phosphatase 2A catalytic subunit favors association with the alpha 4 subunit which promotes dephosphorylation of elongation factor-2. Biochemistry. 1999;38(32): 10371-6. PMID: 10441131
  • Eto M, Karginov A, Brautigan D. A novel phosphoprotein inhibitor of protein type-1 phosphatase holoenzymes. Biochemistry. 1999;38(51): 16952-7. PMID: 10606530
  • Guo C, Wang Y, Brautigan D, Larner J. Histone H1 dephosphorylation is mediated through a radiation-induced signal transduction pathway dependent on ATM. The Journal of biological chemistry. 1999;274(26): 18715-20. PMID: 10373485
  • Beutler K, Pankewycz O, Brautigan D. Equivalent uptake of organic and inorganic zinc by monkey kidney fibroblasts, human intestinal epithelial cells, or perfused mouse intestine. Biological trace element research. 1998;61(1): 19-31. PMID: 9498328
  • Edwards S, Van Dyke T, Van Dyke T, Brautigan D. Chromatographic isolation of PP2A from Limulus lateral eyes. Conventional and small scale methods. Methods in molecular biology (Clifton, N.J.). 1998;93 219-34. PMID: 9664540
  • Kakinoki Y, Somers J, Brautigan D. Multisite phosphorylation and the nuclear localization of phosphatase inhibitor 2-green fluorescent protein fusion protein during S phase of the cell growth cycle. The Journal of biological chemistry. 1998;272(51): 32308-14. PMID: 9405437
  • Liao H, Li Y, Brautigan D, Gundersen G. Protein phosphatase 1 is targeted to microtubules by the microtubule-associated protein Tau. The Journal of biological chemistry. 1998;273(34): 21901-8. PMID: 9705329
  • Wu J, Liu J, Thompson I, Oliver C, Shenolikar S, Brautigan D. A conserved domain for glycogen binding in protein phosphatase-1 targeting subunits. FEBS letters. 1998;439(1): 185-91. PMID: 9849903
  • Phosphatases as partners in signaling networks. Advances in second messenger and phosphoprotein research. 1997;31 113-24. PMID: 9344246
  • Murata K, Hirano K, Villa-Moruzzi E, Hartshorne D, Brautigan D. Differential localization of myosin and myosin phosphatase subunits in smooth muscle cells and migrating fibroblasts. Molecular biology of the cell. 1997;8(4): 663-73. PMID: 9247646 | PMCID: PMC276117
  • Murata K, Wu J, Brautigan D. B cell receptor-associated protein alpha4 displays rapamycin-sensitive binding directly to the catalytic subunit of protein phosphatase 2A. Proceedings of the National Academy of Sciences of the United States of America. 1997;94(20): 10624-9. PMID: 9380685 | PMCID: PMC23426
  • Focus on women. Science (New York, N.Y.). 1996;273(5280): 1325a. PMID: 17792214
  • Dent P, Reardon D, Wood S, Lindorfer M, Graber S, Garrison J, Brautigan D, Sturgill T. Inactivation of raf-1 by a protein-tyrosine phosphatase stimulated by GTP and reconstituted by Galphai/o subunits. The Journal of biological chemistry. 1996;271(6): 3119-23. PMID: 8621710
  • Reardon D, Wood S, Brautigan D, Bell G, Dent P, Sturgill T. Activation of a protein tyrosine phosphatase and inactivation of Raf-1 by somatostatin. The Biochemical journal. 1996;314 401-4. PMID: 8670047 | PMCID: PMC1217062
  • Wu J, Kleiner U, Brautigan D. Protein phosphatase type-1 and glycogen bind to a domain in the skeletal muscle regulatory subunit containing conserved hydrophobic sequence motif. Biochemistry. 1996;35(43): 13858-64. PMID: 8901529
  • Flicking the switches: phosphorylation of serine/threonine protein phosphatases. Seminars in cancer biology. 1995;6(4): 211-7. PMID: 8541516
  • Williams J, Jo H, Hunnicutt R, Brautigan D, McDonald J. Tyrosine phosphorylation of phosphatase inhibitor 2. Journal of cellular biochemistry. 1995;57(3): 415-22. PMID: 7768977
  • Protein phosphatases. Recent progress in hormone research. 1994;49 197-214. PMID: 8146424
  • Belandia B, Brautigan D, Martín-Pérez J. Attenuation of ribosomal protein S6 phosphatase activity in chicken embryo fibroblasts transformed by Rous sarcoma virus. Molecular and cellular biology. 1994;14(1): 200-6. PMID: 8264587 | PMCID: PMC358370
  • Chen J, Parsons S, Brautigan D. Tyrosine phosphorylation of protein phosphatase 2A in response to growth stimulation and v-src transformation of fibroblasts. The Journal of biological chemistry. 1994;269(11): 7957-62. PMID: 7510677
  • Martin B, Shriner C, Brautigan D. Concurrent purification of type-1 and type-2A protein phosphatase catalytic subunits. Protein expression and purification. 1994;5(3): 211-7. PMID: 7950363
  • Toivola D, Eriksson J, Brautigan D. Identification of protein phosphatase 2A as the primary target for microcystin-LR in rat liver homogenates. FEBS letters. 1994;344(2): 175-80. PMID: 8187879
  • Wang L, Orser B, Brautigan D, MacDonald J. Regulation of NMDA receptors in cultured hippocampal neurons by protein phosphatases 1 and 2A. Nature. 1994;369(6477): 230-2. PMID: 8183343
  • Passing or failing? Is biomedical science meeting national needs? FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 1993;7(9): 730-2. PMID: 8330678
  • Brautigan D, Pinault F. Serine phosphorylation of protein tyrosine phosphatase (PTP1B) in HeLa cells in response to analogues of cAMP or diacylglycerol plus okadaic acid. Molecular and cellular biochemistry. 1993;127 121-9. PMID: 7935344
  • Great expectations: protein tyrosine phosphatases in cell regulation. Biochimica et biophysica acta. 1992;1114(1): 63-77. PMID: 1390871
  • Boylan J, Brautigan D, Madden J, Raven T, Ellis L, Gruppuso P. Differential regulation of multiple hepatic protein tyrosine phosphatases in alloxan diabetic rats. The Journal of clinical investigation. 1992;90(1): 174-9. PMID: 1321840 | PMCID: PMC443078
  • Buzzi M, Lu L, Lombardi A, Posner M, Brautigan D, Fast L, Frackelton A. Differentiation-induced changes in protein-tyrosine phosphatase activity and commensurate expression of CD45 in human leukemia cell lines. Cancer research. 1992;52(14): 4027-35. PMID: 1535552
  • Chen J, Martin B, Brautigan D. Regulation of protein serine-threonine phosphatase type-2A by tyrosine phosphorylation. Science (New York, N.Y.). 1992;257(5074): 1261-4. PMID: 1325671
  • DeRemer M, Saeli R, Brautigan D, Edelman A. Ca(2+)-calmodulin-dependent protein kinases Ia and Ib from rat brain. II. Enzymatic characteristics and regulation of activities by phosphorylation and dephosphorylation. The Journal of biological chemistry. 1992;267(19): 13466-71. PMID: 1320022
  • Eriksson J, Brautigan D, Vallee R, Olmsted J, Fujiki H, Goldman R. Cytoskeletal integrity in interphase cells requires protein phosphatase activity. Proceedings of the National Academy of Sciences of the United States of America. 1992;89(22): 11093-7. PMID: 1332069 | PMCID: PMC50490
  • Fernandez A, Brautigan D, Lamb N. Protein phosphatase type 1 in mammalian cell mitosis: chromosomal localization and involvement in mitotic exit. The Journal of cell biology. 1992;116(6): 1421-30. PMID: 1311712 | PMCID: PMC2289383
  • Finn A, Gaido M, Dillard M, Brautigan D. Regulation of an epithelial chloride channel by direct phosphorylation and dephosphorylation. The American journal of physiology. 1992;263(1): C172-5. PMID: 1322039
  • Gordon S, Brautigan D, Zimmerman A. Protein phosphatases modulate the apparent agonist affinity of the light-regulated ion channel in retinal rods. Neuron. 1992;9(4): 739-48. PMID: 1382474
  • Kyriakis J, App H, Zhang X, Banerjee P, Brautigan D, Rapp U, Avruch J. Raf-1 activates MAP kinase-kinase. Nature. 1992;358(6385): 417-21. PMID: 1322500
  • Suganuma M, Fujiki H, Okabe S, Nishiwaki S, Brautigan D, Ingebritsen T, Rosner M. Structurally different members of the okadaic acid class selectively inhibit protein serine/threonine but not tyrosine phosphatase activity. Toxicon : official journal of the International Society on Toxinology. 1992;30(8): 873-8. PMID: 1326138
  • Brautigan D, Pinault F. Activation of membrane protein-tyrosine phosphatase involving cAMP- and Ca2+/phospholipid-dependent protein kinases. Proceedings of the National Academy of Sciences of the United States of America. 1991;88(15): 6696-700. PMID: 1650478 | PMCID: PMC52155
  • Chung S, Reinhart P, Martin B, Brautigan D, Levitan I. Protein kinase activity closely associated with a reconstituted calcium-activated potassium channel. Science (New York, N.Y.). 1991;253(5019): 560-2. PMID: 1857986
  • DeFranco D, Qi M, Borror K, Garabedian M, Brautigan D. Protein phosphatase types 1 and/or 2A regulate nucleocytoplasmic shuttling of glucocorticoid receptors. Molecular endocrinology (Baltimore, Md.). 1991;5(9): 1215-28. PMID: 1663212
  • Gruppuso P, Boylan J, Smiley B, Fallon R, Brautigan D. Hepatic protein tyrosine phosphatases in the rat. The Biochemical journal. 1991;274 361-7. PMID: 1848753 | PMCID: PMC1150145
  • Gruppuso P, Mikumo R, Brautigan D, Braun L. Growth arrest induced by transforming growth factor beta 1 is accompanied by protein phosphatase activation in human keratinocytes. The Journal of biological chemistry. 1991;266(6): 3444-8. PMID: 1847373
  • Kyriakis J, Brautigan D, Ingebritsen T, Avruch J. pp54 microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity. The Journal of biological chemistry. 1991;266(16): 10043-6. PMID: 1645334
  • Martin B, Shriner C, Brautigan D. Modulation of type-1 protein phosphatase by synthetic peptides corresponding to the carboxyl terminus. FEBS letters. 1991;285(1): 6-10. PMID: 1648514
  • Nyomba B, Brautigan D, Schlender K, Wang W, Bogardus C, Mott D. Deficiency in phosphorylase phosphatase activity despite elevated protein phosphatase type-1 catalytic subunit in skeletal muscle from insulin-resistant subjects. The Journal of clinical investigation. 1991;88(5): 1540-5. PMID: 1658044 | PMCID: PMC295665
  • Reinhart P, Chung S, Martin B, Brautigan D, Levitan I. Modulation of calcium-activated potassium channels from rat brain by protein kinase A and phosphatase 2A. The Journal of neuroscience : the official journal of the Society for Neuroscience. 1991;11(6): 1627-35. PMID: 1646298
  • Valentine M, Widmer M, Ledbetter J, Pinault F, Voice R, Clark E, Gallis B, Brautigan D. Interleukin 2 stimulates serine phosphorylation of CD45 in CTLL-2.4 cells. European journal of immunology. 1991;21(4): 913-9. PMID: 1850360
  • Washburn T, Hocutt A, Brautigan D, Korach K. Uterine estrogen receptor in vivo: phosphorylation of nuclear specific forms on serine residues. Molecular endocrinology (Baltimore, Md.). 1991;5(2): 235-42. PMID: 2038345
  • Brautigan D, Sunwoo J, Labbé J, Fernandez A, Lamb N. Cell cycle oscillation of phosphatase inhibitor-2 in rat fibroblasts coincident with p34cdc2 restriction. Nature. 1990;344(6261): 74-8. PMID: 2406614
  • Fernandez A, Brautigan D, Mumby M, Lamb N. Protein phosphatase type-1, not type-2A, modulates actin microfilament integrity and myosin light chain phosphorylation in living nonmuscle cells. The Journal of cell biology. 1990;111(1): 103-12. PMID: 2164027 | PMCID: PMC2116151
  • Gruppuso P, Boylan J, Posner B, Faure R, Brautigan D. Hepatic protein phosphotyrosine phosphatase. Dephosphorylation of insulin and epidermal growth factor receptors in normal and alloxan diabetic rats. The Journal of clinical investigation. 1990;85(6): 1754-60. PMID: 2161429 | PMCID: PMC296637
  • Kovacina K, Yonezawa K, Brautigan D, Tonks N, Rapp U, Roth R. Insulin activates the kinase activity of the Raf-1 proto-oncogene by increasing its serine phosphorylation. The Journal of biological chemistry. 1990;265(21): 12115-8. PMID: 2197270
  • Pallas D, Shahrik L, Martin B, Jaspers S, Miller T, Brautigan D, Roberts T. Polyoma small and middle T antigens and SV40 small t antigen form stable complexes with protein phosphatase 2A. Cell. 1990;60(1): 167-76. PMID: 2153055
  • Brautigan D, Shriner C. Protein phosphatase type 1 catalytic subunit forms nondissociable dimers. Archives of biochemistry and biophysics. 1989;275(1): 44-52. PMID: 2554811
  • Gruppuso P, Brautigan D. Induction of hepatic glycogenesis in the fetal rat. The American journal of physiology. 1989;256(1): E49-54. PMID: 2492154
  • Ishihara H, Martin B, Brautigan D, Karaki H, Ozaki H, Kato Y, Fusetani N, Watabe S, Hashimoto K, Uemura D. Calyculin A and okadaic acid: inhibitors of protein phosphatase activity. Biochemical and biophysical research communications. 1989;159(3): 871-7. PMID: 2539153
  • Picard A, Capony J, Brautigan D, Dorée M. Involvement of protein phosphatases 1 and 2A in the control of M phase-promoting factor activity in starfish. The Journal of cell biology. 1989;109(6): 3347-54. PMID: 2574724 | PMCID: PMC2115967
  • Molecular defects in ion channel regulation in cystic fibrosis predicted from analysis of protein phosphorylation/dephosphorylation. The International journal of biochemistry. 1988;20(8): 745-52. PMID: 2458979
  • Arino J, Woon C, Brautigan D, Miller T, Johnson G. Human liver phosphatase 2A: cDNA and amino acid sequence of two catalytic subunit isotypes. Proceedings of the National Academy of Sciences of the United States of America. 1988;85(12): 4252-6. PMID: 2837763 | PMCID: PMC280405
  • Brautigan D, Kuplic J. Proposal for a pathway to mediate the metabolic effects of insulin. The International journal of biochemistry. 1988;20(4): 349-56. PMID: 2835273
  • Brautigan D, Shriner C. Methods to distinguish various types of protein phosphatase activity. Methods in enzymology. 1988;159 339-46. PMID: 2842599
  • Gruppuso P, Brautigan D. Compartmentalized protein phosphorylation/dephosphorylation in glycogen particles from rabbit skeletal muscle. Biochemistry international. 1988;16(6): 1027-32. PMID: 2845990
  • Roome J, O'Hare T, Pilch P, Brautigan D. Protein phosphotyrosine phosphatase purified from the particulate fraction of human placenta dephosphorylates insulin and growth-factor receptors. The Biochemical journal. 1988;256(2): 493-500. PMID: 2851993 | PMCID: PMC1135437
  • Gruppuso P, Shriner C, Brautigan D. Latent forms of type-1 protein phosphatase in rabbit skeletal muscle. Biochemical and biophysical research communications. 1987;148(3): 1174-81. PMID: 2825677
  • Johnson G, Brautigan D, Shriner C, Jaspers S, Arino J, Mole J, Miller T, Mumby M. Sequence homologies between type 1 and type 2A protein phosphatases. Molecular endocrinology (Baltimore, Md.). 1987;1(10): 745-8. PMID: 2856400
  • Rotenberg S, Brautigan D. Membrane protein phosphotyrosine phosphatase in rabbit kidney. Proteolysis activates the enzyme and generates soluble catalytic fragments. The Biochemical journal. 1987;243(3): 747-54. PMID: 2821989 | PMCID: PMC1147921
  • Shriner C, Brautigan D. Metal chelate affinity chromatography of Zn2+-inhibited protein Tyr(P) phosphatases. Journal of biochemical and biophysical methods. 1987;14(5): 273-8. PMID: 2826566
  • Brautigan D, Gruppuso P, Mumby M. Protein phosphatase type-1 and type-2 catalytic subunits both bind inhibitor-2 and monoclonal immunoglobulins. The Journal of biological chemistry. 1986;261(32): 14924-8. PMID: 3021755
  • Gewirtz H, Olsson R, Brautigan D, Brown P, Most A. Adenosine's role in regulating basal coronary arteriolar tone. The American journal of physiology. 1986;250(6): H1030-6. PMID: 3717357
  • Sparks J, Brautigan D. Molecular basis for substrate specificity of protein kinases and phosphatases. The International journal of biochemistry. 1986;18(6): 497-504. PMID: 3011539
  • Brautigan D, Randazzo P, Shriner C, Fain J. Phosphorylation of chloroform soluble compounds in plasma membranes of human epidermoid carcinoma A431 cells. Lipids. 1985;20(7): 492-5. PMID: 2993780
  • Brautigan D, Shriner C, Gruppuso P. Phosphorylase phosphatase catalytic subunit. Evidence that the Mr = 33,000 enzyme fragment is derived from a native protein of Mr = 70,000. The Journal of biological chemistry. 1985;260(7): 4295-302. PMID: 2984200
  • Gruppuso P, Johnson G, Constantinides M, Brautigan D. Phosphorylase phosphatase regulatory subunit. "Western" blotting with immunoglobulins against inhibitor-2 reveals a protein of Mr = 60,000. The Journal of biological chemistry. 1985;260(7): 4288-94. PMID: 2984199
  • Sparks J, Brautigan D. Specificity of protein phosphotyrosine phosphatases. Comparison with mammalian alkaline phosphatase using polypeptide substrates. The Journal of biological chemistry. 1985;260(4): 2042-5. PMID: 2982803
  • Shriner C, Brautigan D. Cytosolic protein phosphotyrosine phosphatases from rabbit kidney. Purification of two distinct enzymes that bind to Zn2+-iminodiacetate agarose. The Journal of biological chemistry. 1984;259(18): 11383-90. PMID: 6088542
  • Ballou L, Brautigan D, Fischer E. Subunit structure and activation of inactive phosphorylase phosphatase. Biochemistry. 1983;22(14): 3393-9. PMID: 6311247
  • Gewirtz H, Brautigan D, Olsson R, Brown P, Most A. Role of adenosine in the maintenance of coronary vasodilation distal to a severe coronary artery stenosis. Observations in conscious domestic swine. Circulation research. 1983;53(1): 42-51. PMID: 6861296