Brian H. Annex


  • MD, Yale School of Medicine
  • Residency, Tufts University School of Medicine
  • Clinical Fellowship, Duke University School of Medicine, Durham, NC
  • Clinical Fellowship, William Beaumont Hospital

Primary Appointment

  • Professor, Medicine- Cardiovascular Medicine


Research Interest(s)

Angiogenesis; peripheral arterial disease (PAD); gene therapy

Research Description

Dr. Annex's clinical and research interests include a multidisciplinary program in angiogenesis (the growth of new blood vessels) that is focused mainly on peripheral arterial disease (PAD), in which blockages in arteries cause a series of clinical problems. His group is involved in a number of bench-to-bedside studies, including investigations of the causes of blockages in arteries outside the heart, in case of the legs the response of the muscle and new arterial growth that follow the blockages, and the impact that diabetes mellitus plays in PAD. Included among the array studies are gene or cell therapy clinical investigation to promote blood vessel growth in patients with PAD, as well as studies of exercise training, and pre-clinical models of PAD.

Selected Publications

  • Dokun A, Chen L, Lanjewar S, Lye R, Annex B. Glycaemic control improves perfusion recovery and VEGFR2 protein expression in diabetic mice following experimental PAD. Cardiovascular research. 2014;101(3): 364-72. PMID: 24385342 | PMCID: PMC3928005
  • French B, Annex B. AAV9 and Cre: a one-two punch for a quick cardiac knockout. Cardiovascular research. 2014. PMID: 25187523
  • Meisner J, Annex B, Price R. Despite normal arteriogenic and angiogenic responses, hind limb perfusion recovery and necrotic and fibroadipose tissue clearance are impaired in matrix metalloproteinase 9-deficient mice. Journal of vascular surgery. 2014. PMID: 24582703 | PMCID: PMC4147032
  • Naresh N, Chen X, Roy R, Antkowiak P, Annex B, Epstein F. Accelerated dual-contrast first-pass perfusion MRI of the mouse heart: Development and application to diet-induced obese mice. Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine. 2014. PMID: 24760707
  • Nehler M, Duval S, Diao L, Annex B, Hiatt W, Rogers K, Zakharyan A, Hirsch A. Epidemiology of peripheral arterial disease and critical limb ischemia in an insured national population. Journal of vascular surgery. 2014;60(3): 686-695.e2. PMID: 24820900
  • Okello S, Millard A, Owori R, Asiimwe S, Siedner M, Rwebembera J, Wilson L, Moore C, Annex B. Prevalence of lower extremity peripheral artery disease among adult diabetes patients in southwestern Uganda. BMC cardiovascular disorders. 2014;14 75. PMID: 24913468 | PMCID: PMC4057935
  • Okutsu M, Call J, Lira V, Zhang M, Donet J, French B, Martin K, Peirce-Cottler S, Rembold C, Annex B, Yan Z. Extracellular superoxide dismutase ameliorates skeletal muscle abnormalities, cachexia, and exercise intolerance in mice with congestive heart failure. Circulation. Heart failure. 2014;7(3): 519-30. PMID: 24523418 | PMCID: PMC4080303
  • Allen J, Stabler T, Kenjale A, Ham K, Robbins J, Duscha B, Kraus W, Annex B. Diabetes status differentiates endothelial function and plasma nitrite response to exercise stress in peripheral arterial disease following supervised training. Journal of diabetes and its complications. 2013;28(2): 219-25. PMID: 24355663 | PMCID: PMC3943470
  • Meisner J, Song J, Annex B, Price R. Myoglobin overexpression inhibits reperfusion in the ischemic mouse hindlimb through impaired angiogenesis but not arteriogenesis. The American journal of pathology. 2013;183(6): 1710-8. PMID: 24095922 | PMCID: PMC5746951
  • Mohler E, Hiatt W, Gornik H, Kevil C, Quyyumi A, Haynes W, Annex B. Sodium nitrite in patients with peripheral artery disease and diabetes mellitus: safety, walking distance and endothelial function. Vascular medicine (London, England). 2013;19(1): 9-17. PMID: 24363302