Barbara J. Mann

Education

  • PhD, University of Virginia

Primary Appointment

  • Associate Professor, Medicine- Infectious Diseases and International Health

Contact

Research Interest(s)

Pathogenicity of Francisella tularensis and vaccines

Research Description

Research Interests

My research interests focus on the initial interactions between the pathogenic bacteria Francisella tularensis and its host. I am specifically interested in adherence and invasion mechanisms, and protective vaccines. F. tularensis has been designated as a category A select agent for studies in Biodefense Research.

Francisella tularensis is a zoonotic infection that can be contracted by several methods including tick bites and inhalation. The low infection dose of 10-50 organisms by an inhalation route led to its development as an agent of biological warfare. The molecular mechanisms of pathogenicity of this gram negative, intracellular bacteria are largely unknown. We are using combined approaches that include bioinformatics, and mutagenesis to identify proteins that mediate adherence to and invasion of host cells. We are also screening for attenuated strains that may be useful as protective vaccines.

Selected Publications

  • Reed D, Smith L, Cole K, Santiago A, Mann B, Barry E. Live attenuated mutants of Francisella tularensis protect rabbits against aerosol challenge with a virulent type A strain. Infection and immunity. 2014. PMID: 24614653
  • Chong A, Child R, Wehrly T, Rockx-Brouwer D, Qin A, Mann B, Celli J. Structure-Function Analysis of DipA, a Francisella tularensis Virulence Factor Required for Intracellular Replication. PloS one. 2013;8(6): e67965. PMID: 23840797
  • Moreau G, Mann B. Adherence and uptake of Francisella into host cells. Virulence. 2013;4(8): 826-32. PMID: 23921460
  • Richard K, Mann B, Stocker L, Barry E, Qin A, Cole L, Hurley M, Ernst R, Michalek S, Stein D, Deshong P, Vogel S. Novel Catanionic Surfactant Vesicle Vaccines Protect Mice against Francisella tularensis LVS and Confer Significant Partial Protection against F. tularensis Schu S4. Clinical and vaccine immunology : CVI. 2013. PMID: 24351755
  • Ark N, Mann B. Impact of Francisella tularensis pilin homologs on pilus formation and virulence. Microbial pathogenesis. 2011;51(3): 110-20. PMID: 21605655
  • Cole L, Mann B, Shirey K, Richard K, Yang Y, Gearhart P, Chesko K, Viscardi R, Vogel S. Role of TLR signaling in Francisella tularensis-LPS-induced, antibody-mediated protection against Francisella tularensis challenge. Journal of leukocyte biology. 2011;90(4): 787-97. PMID: 21750122
  • Qin A, Scott D, Rabideau M, Moore E, Mann B. Requirement of the CXXC motif of novel Francisella infectivity potentiator protein B FipB, and FipA in virulence of F. tularensis subsp. tularensis. PloS one. 2011;6(9): e24611. PMID: 21931773
  • Singh U, Gilchrist C, Schaenman J, Rogers J, Hockensmith J, Mann B, Petri W. Context-dependent roles of the Entamoeba histolytica core promoter element GAAC in transcriptional activation and protein complex assembly. Molecular and biochemical parasitology. 2002;120(1): 107-16. PMID: 11849710
  • Schaenman J, Driscoll P, Hockensmith J, Mann B, Petri W. An upstream regulatory element containing two nine basepair repeats regulates expression of the Entamoeba histolytica hgl5 lectin gene. Molecular and biochemical parasitology. 1998;94(2): 309-13. PMID: 9747982