Barbara J. Mann

Education

  • PhD, University of Virginia

Primary Appointment

  • Associate Professor, Medicine- Infectious Diseases and International Health

Contact

Research Interest(s)

Pathogenicity of Francisella tularensis and vaccines

Research Description

Research Interests My research interests focus on the initial interactions between the pathogenic bacteria Francisella tularensis and its host. I am specifically interested in adherence and invasion mechanisms, and protective vaccines. F. tularensis has been designated as a category A select agent for studies in Biodefense Research.

Francisella tularensis is a zoonotic infection that can be contracted by several methods including tick bites and inhalation. The low infection dose of 10-50 organisms by an inhalation route led to its development as an agent of biological warfare. The molecular mechanisms of pathogenicity of this gram negative, intracellular bacteria are largely unknown. We are using combined approaches that include bioinformatics, and mutagenesis to identify proteins that mediate adherence to and invasion of host cells. We are also screening for attenuated strains that may be useful as protective vaccines.

Selected Publications

  • Gorla S, Zhang Y, Rabideau M, Qin A, Chacko S, House A, Johnson C, Mandapati K, Bernstein H, McKenney E, Boshoff H, Zhang M, Glomski I, Goldberg J, Cuny G, Mann B, Hedstrom L. Benzoxazoles, Phthalazinones, and Arylurea-Based Compounds with IMP Dehydrogenase-Independent Antibacterial Activity against Francisella tularensis. Antimicrobial agents and chemotherapy. 2017;61(10). PMID: 28739786 | PMCID: PMC5610529
  • Moreau G, Qin A, Mann B. Zinc Acquisition Mechanisms Differ between Environmental and Virulent Francisella Species. Journal of bacteriology. 2017;200(4). PMID: 29109188 | PMCID: PMC5786701
  • Richard K, Mann B, Qin A, Barry E, Ernst R, Vogel S. Monophosphoryl Lipid A Enhances Efficacy of a Francisella tularensis LVS-Catanionic Nanoparticle Subunit Vaccine against F. tularensis Schu S4 Challenge by Augmenting both Humoral and Cellular Immunity. Clinical and vaccine immunology : CVI. 2017;24(3). PMID: 28077440 | PMCID: PMC5339645
  • Qin A, Zhang Y, Clark M, Moore E, Rabideau M, Moreau G, Mann B. Components of the type six secretion system are substrates of Francisella tularensis Schu S4 DsbA-like FipB protein. Virulence. 2016;7(8): 882-894. PMID: 27028889 | PMCID: PMC5160417
  • Santiago A, Mann B, Qin A, Cunningham A, Cole L, Grassel C, Vogel S, Levine M, Barry E. Characterization of Francisella tularensis Schu S4 defined mutants as live-attenuated vaccine candidates. Pathogens and disease. 2015;73(6): ftv036. PMID: 25986219 | PMCID: PMC4462183
  • Qin A, Zhang Y, Clark M, Rabideau M, Millan Barea L, Mann B. FipB, an Essential Virulence Factor of Francisella tularensis subsp. tularensis, Has Dual Roles in Disulfide Bond Formation. Journal of bacteriology. 2014;196(20): 3571-81. PMID: 25092026
  • Reed D, Smith L, Cole K, Santiago A, Mann B, Barry E. Live attenuated mutants of Francisella tularensis protect rabbits against aerosol challenge with a virulent type A strain. Infection and immunity. 2014. PMID: 24614653
  • Chong A, Child R, Wehrly T, Rockx-Brouwer D, Qin A, Mann B, Celli J. Structure-Function Analysis of DipA, a Francisella tularensis Virulence Factor Required for Intracellular Replication. PloS one. 2013;8(6): e67965. PMID: 23840797 | PMCID: PMC3694160
  • Moreau G, Mann B. Adherence and uptake of Francisella into host cells. Virulence. 2013;4(8): 826-32. PMID: 23921460 | PMCID: PMC3925714
  • Richard K, Mann B, Stocker L, Barry E, Qin A, Cole L, Hurley M, Ernst R, Michalek S, Stein D, Deshong P, Vogel S. Novel Catanionic Surfactant Vesicle Vaccines Protect Mice against Francisella tularensis LVS and Confer Significant Partial Protection against F. tularensis Schu S4. Clinical and vaccine immunology : CVI. 2013. PMID: 24351755 | PMCID: PMC3910930
  • Ark N, Mann B. Impact of Francisella tularensis pilin homologs on pilus formation and virulence. Microbial pathogenesis. 2011;51(3): 110-20. PMID: 21605655 | PMCID: PMC3120926
  • Cole L, Mann B, Shirey K, Richard K, Yang Y, Gearhart P, Chesko K, Viscardi R, Vogel S. Role of TLR signaling in Francisella tularensis-LPS-induced, antibody-mediated protection against Francisella tularensis challenge. Journal of leukocyte biology. 2011;90(4): 787-97. PMID: 21750122 | PMCID: PMC3177696
  • Qin A, Scott D, Rabideau M, Moore E, Mann B. Requirement of the CXXC motif of novel Francisella infectivity potentiator protein B FipB, and FipA in virulence of F. tularensis subsp. tularensis. PloS one. 2011;6(9): e24611. PMID: 21931773 | PMCID: PMC3169626
  • Ahmad S, Hunter L, Qin A, Mann B, van Hoek M. Azithromycin effectiveness against intracellular infections of Francisella. BMC microbiology. 2010;10 123. PMID: 20416090 | PMCID: PMC2881020
  • Gilchrist C, Moore E, Zhang Y, Bousquet C, Lannigan J, Mann B, Petri W. Regulation of Virulence of Entamoeba histolytica by the URE3-BP Transcription Factor. mBio. 2010;1(1). PMID: 20689746 | PMCID: PMC2912668
  • Mann B, Ark N. Rationally designed tularemia vaccines. Expert review of vaccines. 2009;8(7): 877-85. PMID: 19538114 | PMCID: PMC2748800
  • Qin A, Scott D, Mann B. Francisella tularensis subsp. tularensis Schu S4 disulfide bond formation protein B, but not an RND-type efflux pump, is required for virulence. Infection and immunity. 2008;76(7): 3086-92. PMID: 18458069 | PMCID: PMC2446700
  • Qin A, Scott D, Thompson J, Mann B. Identification of an essential Francisella tularensis subsp. tularensis virulence factor. Infection and immunity. 2008;77(1): 152-61. PMID: 18981253 | PMCID: PMC2612291
  • Qin A, Mann B. Identification of transposon insertion mutants of Francisella tularensis tularensis strain Schu S4 deficient in intracellular replication in the hepatic cell line HepG2. BMC microbiology. 2006;6 69. PMID: 16879747 | PMCID: PMC1557513
  • Loftus B, Anderson I, Davies R, Alsmark U, Samuelson J, Amedeo P, Roncaglia P, Berriman M, Hirt R, Mann B, Nozaki T, Suh B, Pop M, Duchene M, Ackers J, Tannich E, Leippe M, Hofer M, Bruchhaus I, Willhoeft U, Bhattacharya A, Chillingworth T, Churcher C, Hance Z, Harris B, Harris D, Jagels K, Moule S, Mungall K, Ormond D, Squares R, Whitehead S, Quail M, Rabbinowitsch E, Norbertczak H, Price C, Wang Z, Guillén N, Gilchrist C, Stroup S, Bhattacharya S, Lohia A, Foster P, Sicheritz-Ponten T, Weber C, Singh U, Mukherjee C, El-Sayed N, Petri W, Clark C, Embley T, Barrell B, Fraser C, Hall N. The genome of the protist parasite Entamoeba histolytica. Nature. 2005;433(7028): 865-8. PMID: 15729342