- Biomedical Sciences Graduate Program | Andreas Gahlmann

Andreas Gahlmann

Education

  • Postdoc, Stanford University
  • PhD, California Institute of Technology
  • BA,BS, University of Portland

Contact

Research Interest(s)

Bacterial cell biology at the molecular level

Research Description

We develop nanoscale imaging methods for 3D single-molecule localization in intact bacterial cells. Our two primary research objectives are:

1. Development of 3D super-resolution imaging methods, including instrument design, sample preparation, and computational image analysis.

2. Application of these methods to understand molecular-level spatial and temporal phenomena inside intact bacterial cells and cellular-level interactions within developing microbial communities. To achieve these objectives, we operate in a multidisciplinary research environment that integrates aspects from several areas of chemistry, molecular and cellular biology, as well as biophysics, optical physics, engineering, and computer science.

Our experiments access the molecular (nanometers) length scales that are inaccessible with conventional diffraction-limited fluorescence microscopy and bridge to the cellular (micrometers), and intercellular (10-100 micrometers) length scales. Utilizing the single-molecule sensitivity and specificity of super-resolution fluorescence microscopy, we can localize single biomolecules or single cells in 3D space and track their motion over time. Wherever possible, we primarily perform imaging experiments with living cells to characterize how molecular- or cellular-level spatial and temporal phenomena determine the physiology and phenotype of the cell or the functionalities of entire cell populations. Projects in the lab address a variety of biological processes with direct relevance to bacterial pathogenesis and biotechnological applications.

Selected Publications

  • Ptacin J, Gahlmann A, Bowman G, Perez A, von Diezmann A, Eckart M, Moerner W, Shapiro L. Bacterial scaffold directs pole-specific centromere segregation. Proceedings of the National Academy of Sciences of the United States of America. 2014;111(19): E2046-55. PMID: 24778223 | PMCID: PMC4024888
  • Gahlmann A, Moerner W. Exploring bacterial cell biology with single-molecule tracking and super-resolution imaging. Nature reviews. Microbiology. 2013;12(1): 9-22. PMID: 24336182 | PMCID: PMC3934628
  • Gahlmann A, Ptacin J, Grover G, Quirin S, von Diezmann A, Lee M, Backlund M, Shapiro L, Piestun R, Moerner W. Quantitative multicolor subdiffraction imaging of bacterial protein ultrastructures in three dimensions. Nano letters. 2013;13(3): 987-93. PMID: 23414562 | PMCID: PMC3599789
  • Lee I, Gahlmann A, Zewail A. Structural dynamics of free amino acids in diffraction. Angewandte Chemie (International ed. in English). 2011;51(1): 99-102. PMID: 22086765
  • Gahlmann A, Lee I, Zewail A. Direct structural determination of conformations of photoswitchable molecules by laser desorption-electron diffraction. Angewandte Chemie (International ed. in English). 2010;49(37): 6524-7. PMID: 20715254
  • Gahlmann A, Park S, Zewail A. Structure of isolated biomolecules by electron diffraction-laser desorption: uracil and guanine. Journal of the American Chemical Society. 2008;131(8): 2806-8. PMID: 19055399
  • Gahlmann A, Tae Park S, Zewail A. Ultrashort electron pulses for diffraction, crystallography and microscopy: theoretical and experimental resolutions. Physical chemistry chemical physics : PCCP. 2008;10(20): 2894-909. PMID: 18473038
  • Park S, Gahlmann A, He Y, Feenstra J, Zewail A. Ultrafast electron diffraction reveals dark structures of the biological chromophore indole. Angewandte Chemie (International ed. in English). 2008;47(49): 9496-9. PMID: 18988218
  • He Y, Gahlmann A, Feenstra J, Park S, Zewail A. Ultrafast electron diffraction: structural dynamics of molecular rearrangement in the NO release from nitrobenzene. Chemistry, an Asian journal. 2007;1(1): 56-63. PMID: 17441038
  • Gahlmann A, Kester K, Mayer S. Investigating the effect of the zwitterion/lactone equilibrium of rhodamine B on the cybotactic region of the acetonitrile/scCO2 cosolvent. The journal of physical chemistry. A. 2006;109(9): 1753-7. PMID: 16833502